- decaprenyl-phosphate glcnac-1-phosphate transferase
- arabinose phosphate isomerase
- imidazoleglycerol-phosphate dehydratase
- carbamoyl-phosphate synthase (h353n)
- glucose-1-phosphate thymidylyltransferase
- glucose-6-phosphate dehydrogenase
- glyceraldehyde-3-phosphate dehydrogenase
- mannose-6-phosphate isomerase
- orotidine 5'-phosphate decarboxylase
- orotidine 5-phosphate decarboxylase
- orotidine-5'-phosphate decarboxylase
- sphingosine 1-phosphate receptor
- sphingosine-1-phosphate aldolase
- trehalose-6-phosphate phosphatase
- 1-deoxyxylulose-5-phosphate reductoisomerase
- arabinose 5-phosphate isomerase kdsd
- glucosamine-1-phosphate n-acetyltransferase
- glucose 6-phosphate dehydrogenase (g6pd)
- glucose-6-phosphate 1-dehydrogenase
- glyceraldehyde-3-phosphate dehydrogenase liver
- glyceraldehyde-3-phosphate dehydrogenase, cytosolic
- n-acetylglucosamine-1-phosphate uridyltransferase
- phosphate regulon sensor protein phor
- sodium/inorganic phosphate cotransporter iic
- sphingosine 1-phosphate receptor 1
- sphingosine 1-phosphate receptor 2
- sphingosine 1-phosphate receptor 3
- sphingosine 1-phosphate receptor 4
- sphingosine 1-phosphate receptor 5
- sphingosine 1-phosphate receptor gpr6
- sphingosine-1-phosphate receptor 1
- 1-phosphatidylinositol 3-phosphate 5-kinase
- f420-dependent glucose-6-phosphate dehydrogenase
- glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase
- glutamine--fructose-6-phosphate aminotransferase [isomerizing]
- glutamine--fructose-6-phosphate transaminase (isomerizing)
- renal sodium-phosphate transport protein 1
- sodium-dependent phosphate transport protein 2a
- sodium-dependent phosphate transport protein 2b
- sodium-dependent phosphate transport protein 2c
- sphingosine 1-phosphate receptor 1 (s1p1)
- sphingosine 1-phosphate receptor 2 (s1p2)
- sphingosine 1-phosphate receptor edg-1
- sphingosine 1-phosphate receptor edg-5
- 1-deoxy-d-xylulose 5-phosphate reductoisomerase
- 1-deoxy-d-xylulose-5-phosphate synthase
- f420-dependent glucose-6-phosphate dehydrogenase [w44a]
- f420-dependent glucose-6-phosphate dehydrogenase [w44f]
- f420-dependent glucose-6-phosphate dehydrogenase [w44y]
- glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1
- 1-acyl-sn-glycerol-3-phosphate acyltransferase beta
- 1-deoxy-d-xylulose 5-phosphate reductoisomerase, chloroplastic
- 2-c-methyl-d-erythritol 4-phosphate synthase
- phosphatidylinositol 4-phosphate 5-kinase type i gamma
- phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
- f420-dependent glucose-6-phosphate dehydrogenase mutant (fgd e109q)
- f420-dependent glucose-6-phosphate dehydrogenase mutant (fgd h40a)
- (1->4)-alpha-d-glucan:maltose-1-phosphate alpha-d-maltosyltransferase
- 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase, phe-sensitive
- phosphatidylinositol 4-phosphate 3-kinase c2 domain-containing subunit alpha
- gag-pol polyprotein [600-1159,y780c]/imidazoleglycerol-phosphate dehydratase [600-1029,y780c]
- 1-phosphatidylinositol 3-phosphate 5-kinase [1-2098,s696n,l932s,q995l,t998s,s1033a,q1183k]
Fludarabine Phosphate Fludarabine Fludara BDBM50248004 CHEBI:63599 Oforta
Carbamyl phosphate BDBM50162819 CHEMBL369105 carbamoyl phosphate carbamic phosphoric monoanhydride monocarbamoyl phosphate aminocarbonyl dihydrogen phosphate carbamoyl dihydrogen phosphate
4-Nitrophenyl phosphate disodium salt hexahydrate 4-nitrophenyl phosphate (pNPP) para-nitrophenyl phosphate (pNPP) BDBM13466 disodium (4-nitrophenyl) phosphate
Na2HPO4 disodium monohydrogen phosphate disodium hydrogen phosphate Disodium phosphate disodium acid orthophosphate BDBM50080995 disodium hydrogenphosphate Dibasic sodium phosphate CHEMBL1060
BDBM50422306 TRICIRIBINE PHOSPHATE Triciribine Phosphate Salt Of Tricyclic Nucleoside
Riboflavin 5''-Phosphate BDBM50421345 RIBOFLAVIN 5'-PHOSPHATE E101a
sodium dihydrogen phosphate NaH2PO4 monosodium phosphate CHEMBL1368 BDBM50155534 sodium phosphate, monobasic sodium dihydrogenphosphate sodium phosphate monobasic anhydrous phosphoric acid, monosodium salt
Photrexa Riboflavin 5''-phosphate sodium RIBOFLAVIN 5'-PHOSPHATE SODIUM Phosphated riboflavin Riboflavin sodium phosphate BDBM50523758
BDBM50292724 arabinouridine 3'-phosphate CHEMBL460901 URACIL ARABINOSE-3'-PHOSPHATE
(2R,3S,4S,5R)-2-(6-azanyl-2-fluoranyl-purin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol BDBM68391 MLS000028687 SMR000058874 cid_657237 (2R,3S,4S,5R)-2-(6-amino-2-fluoro-purin-9-yl)-5-methylol-tetrahydrofuran-3,4-diol FLUDARABINE PHOSPHATE FLUDARABINE fludara (2R,3S,4S,5R)-2-(6-amino-2-fluoropurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol (2R,3S,4S,5R)-2-(6-amino-2-fluoro-9-purinyl)-5-(hydroxymethyl)oxolane-3,4-diol
2-amino-ethanol dihydrogen phosphate O-phosphocolamine ethanolamine O-phosphate OPE phosphonoethanolamine monoaminoethyl phosphate colaminphosphoric acid 2-amino-ethanol phosphate PEA colamine phosphate mono(2-aminoethyl) phosphate colamine phosphoric acid 2-aminoethyl dihydrogen phosphate phosphoric acid 2-aminoethyl phenyl ester CHEMBL146972 BDBM50281572 EAP ethanolamine acid phosphate pEtN O-phosphoethanolamine
BDBM241975 Clindamycin phosphate
Homoserine phosphate BDBM92999
p-nitrophenyl phosphate BDBM24514 CHEMBL24231 4-nitrophenyl phosphate 4-nitrophenoxyphosphonic acid
4-Methylumbelliferyl phosphate BDBM213237
BDBM235670 Dexamethasone sodium phosphate
CHEMBL1756 BDBM50333645 Estramustine phosphate
Estradiol phosphate BDBM50333647 CHEMBL1642763
Phosphate analogue, 6 BDBM84557
Phosphate analogue, 9 BDBM84558
mannose 6-phosphate BDBM50275807
sodium phosphate, tribasic tertiaeres Natriumphosphat(V) tribasic sodium orthophosphate sodium orthophosphate CHEMBL363100 phosphoric acid trisodium salt sodium phosphate tertiary sodium phosphate tribasic sodium phosphate BDBM50155537 Na3PO4 Trinatriumphosphat
BDBM50118216 pyridoxal 5'-phosphate CHEMBL82202 (4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate pyridoxal 5'-(dihydrogen phosphate)
BDBM197174 Glucose-6-phosphate (G6P)
BDBM231698 p-Nitrophenyl phosphate (pNPP)
BDBM84559 Phosphate analogue, (s)-14
BDBM84560 Phosphate analogue, (S)-21
para-nitrophenyl phosphate (pNPP) BDBM26100
BDBM50304360 BENZENE-1,2,3,4-TETRAYL TETRAKIS[DIHYDROGEN (PHOSPHATE)] benzene-1,2,3,4-tetrayl tetrakis(hydrogen phosphate) CHEMBL595349
phosphacol p-nitrophenyl diethyl phosphate diethyl p-nitrophenyl phosphate O,O-diethyl O-p-nitrophenyl phosphate CHEMBL23838 diethyl 4-nitrophenyl phosphate diethyl paraoxon paraoxon BDBM50240416 ethyl paraoxon phosphoric acid diethyl 4-nitrophenyl ester
2S,3R-dihydroxybutyramide 4-phosphate BDBM50153711
CHEBI:63036 BDBM50004328 POTASSIUM PHOSPHATE, MONOBASIC
CHEMBL186043 BDBM50155535 Carbamoyl phosphate; di sodium
CHLOROQUINE DIPHOSPHATE CHLOROQUINE PHOSPHATE BDBM50411863 ARALEN
Cytidine 2,3-cyclic phosphate, 6 BDBM31909
Fragment 1 Phosphate phosphoric acid BDBM14671
RUXOLITINIB PHOSPHATE BDBM50391992 INCB018424 SALT Jakafi
4-{hydroxy[(phosphonooxy)acetyl]amino}butyl dihydrogen phosphate N-(4-hydroxybutyl)-glycolohydroxamicacid bis-phosphate CHEMBL1236228 BDBM50330433
BDBM16190 [3-(2-cyanoacetyl)phenyl] diethyl phosphate 3-(2-Cyanoacetyl)phenyl Diethyl Phosphate beta-ketonitrile 5h
DDVP CHEMBL167911 2,2-Dichloroethenyl dimethyl phosphate Dimethyl-2,2-dichlorovinyl phosphate Phosphoric acid, 2,2-dichloroethenyl dimethyl ester Dimethyl 2,2-dichlorovinyl phosphate BDBM50286926 Phosphoric acid, 2,2-dichlorovinyl dimethyl ester
AMP adenosine 5'-phosphate group [5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate BDBM92538
1-deoxy-L-ribulose 5-phosphate BDBM50153712
4S-hydroxypentan-2-one 5-phosphate BDBM50153714
BDBM50153710 3S-hydroxypentan-2-one 5-phosphate
BDBM92531 DiFMUP (6,8-Difluoro-4-methylumbelliferyl phosphate)
CHEMBL1230617 8-Bromo-Adenosine Mono Phosphate BDBM50222467
CHEMBL1275924 Phenyl-N-(4-aminopiperidine)phosphate BDBM50330376
CHEMBL1775011 8-phenyl-Adenosine Mono Phosphate BDBM50343897
US10676467, Compound S1P (2S,3R,4E)-2-amino-4-octadecene-1,3-diol 1-(dihydrogen phosphate) (2S,3R,4E)-2-amino-3-hydroxyoctadec-4-en-1-yl dihydrogen phosphate BDBM50158348 CHEMBL225155 sphingosine-1-phosphate US11584726, Example S1P sphingosine 1-phosphate
sphingosine-1-phosphate, 33P labeled {[(4Z)-2-amino-3-hydroxyoctadec-4-en-1-yl]oxy}phosphonic acid S1P [32P]S1P [33P]S1P BDBM22202 sphingosine-1-phosphate sphingosine-1-phosphate, 32P labeled
2-(methylsulfonamido)-2-oxoethyl dihydrogen phosphate BDBM50175714 CHEMBL370845
BDBM50300123 2-(4-Formylphenoxy)ethyl dimethyl phosphate CHEMBL574442
BDBM50300582 CHEMBL573979 Dibutyl 2,2,2-trifluoro-1-phenylethyl phosphate
CHEBI:83326 TEDIZOLID PHOSPHATE BDBM50017198 TR-701-FA
CHEMBL575281 Dimethyl 2,2,2-trifluoro-1-phenylethyl phosphate BDBM50300580
CHEMBL575301 Diethyl 2,2,2-trifluoro-1-phenylethyl phosphate BDBM50300581
CHEMBL606220 CHEMBL1230617 BDBM50367001 8-Bromo-Adenosine Mono Phosphate
Hexadecyl[2-(N-Methylpiperidinio)ethyl]phosphate hexadecyl 2-(1-methylpiperidinium-1-yl)ethyl phosphate BDBM50051816 (N-methylpiperidino)ethanol hexadecyl phosphonate CHEMBL89618
1,2-dideoxy-L-threo-3-hexulose 6-phosphate BDBM50153715
2-Methoxy-4-(nonanamidomethyl)phenyl dihydrogen phosphate (3b) BDBM165191
BDBM165193 4-(Nonanamidomethyl)-1,2-phenylene bis(dihydrogen phosphate) (6b)
BDBM50175712 CHEMBL199843 2-oxo-2-(phenylsulfonamido)ethyl dihydrogen phosphate
CHEMBL202243 BDBM50176400 (R)-2,3-bis(octyloxy)propyl dihydrogen phosphate
Etopophos preservative free Etoposide Phosphate BMY-40481 Etopophos BDBM50247889
TCP CAS_78-30-8 BDBM82063 TRI-O-CRESYL PHOSPHATE
beta-nicotinamide adenine dinucleotide phosphate, oxidized form NADP+ BDBM11939
Di-Sodium salt of Phosphoric acid mono-{2-methoxy-5-[2-(3,4,5-trimethoxy-phenyl)-vinyl]-phenyl} ester Disodium Phosphoric acid mono-{2-methoxy-5-[2-(3,4,5-trimethoxy-phenyl)-vinyl]-phenyl} ester combretastatin A-4 sodium phosphate BDBM50064259 Phosphoric acid mono-{2-methoxy-5-[(Z)-2-(3,4,5-trimethoxy-phenyl)-vinyl]-phenyl} ester; disodium salt sodium(Z)-2-methoxy-5-(3,4,5-trimethoxystyryl)phenyl phosphate combretastatin A-4 phosphate Combretastatin A4 phosphate disodium 2-methoxy-5-[(Z)-2-(3,4,5-trimethoxyphenyl)vinyl]phenyl phosphate CHEMBL289351 sodium (Z)-5-(3,4,5-trimethoxystyryl)-2-methoxyphenyl phosphate disodium mono(Z)-5-(3,4,5-trimethoxystyryl)-2-methoxyphenyl phosphate
2-[(3-bromophenyl)carbamoyl]-5-chlorophenyl diethyl phosphate (20) BDBM150740
2-[(4-bromophenyl)carbamoyl]-5-chlorophenyl diethyl phosphate (23) BDBM150743
BDBM50032981 Phosphate analogue, 8 (3-Amino-propyl)-phosphonic acid CHEMBL286077
BDBM50247086 Chlorphrifos oxon diethyl 3,5,6-trichloropyridin-2-yl phosphate CHEMBL444970
CHEMBL1235276 BDBM50330438 3-{hydroxy[(phosphonooxy)acetyl]amino}propyl dihydrogen phosphate
Phosphoglycolohydroxamic Acid BDBM50167777 2-(hydroxyamino)-2-oxoethyl dihydrogen phosphate CHEMBL371668
2-amino-4,5-dihydronaphtho[1,2-d][1,3]thiazol-8-yl dihydrogen phosphate 2-amino-4,5-dihydronaphtho[1,2-d]thiazol-8-yl dihydrogen phosphate BDBM50302495 CHEMBL565809
cori ester glucose-1-phosphate alpha-glucose-1-phosphate BDBM23188 {[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy}phosphonic acid
2-(hydroxy(3-hydroxypropyl)amino)-2-oxoethyl hydrogen phosphate BDBM50330436 CHEMBL1276292
2-Methoxy-4-((8-methylnonanamido)methyl)phenyl dihydrogen phosphate (3a) BDBM165190
3-Nitro-1-(beta-D-ribofuranosyl)pyrrole 5'-phosphate BDBM50247810 CHEMBL538120
4-Nitro-1-(-beta-D-ribofuranosyl)pyrazole 5'-phosphate BDBM50247822 CHEMBL538121
5-chloro-2-[(3-chlorophenyl)carbamoyl]phenyl diethyl phosphate (19) BDBM150739
5-chloro-2-[(3-fluorophenyl)carbamoyl]phenyl diethyl phosphate (21) BDBM150741
5-chloro-2-[(4-fluorophenyl)carbamoyl]phenyl diethyl phosphate (22) BDBM150742
5-formyl-6-hydroxy-2-naphthyl di-sodium phosphate BDBM50183272 CHEMBL382634
BDBM150744 5-chloro-2-[(4-chlorophenyl)carbamoyl]phenyl diethyl phosphate (24)
BDBM150745 5-chloro-2-[(3,4-dichlorophenyl)carbamoyl]phenyl diethyl phosphate (25)
BDBM165192 4-((8-Methylnonanamido)methyl)-1,2-phenylene bis(dihydrogen phosphate) (6a)
BDBM50247808 CHEMBL538119 3-Nitro-1-(beta-D-ribofuranosyl)imidazole 5'-phosphate
CHEBI:68603 Betamethasone phosphoric acid BETAMETHASONE PHOSPHORIC ACID BDBM50613630 Betamethasone phosphate
[5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl hydrogen phosphate;triethylazanium SMR000718807 [5-(6-aminopurin-9-yl)-3,4-dihydroxy-2-oxolanyl]methyl hydrogen phosphate;triethylammonium (5-adenin-9-yl-3,4-dihydroxy-tetrahydrofuran-2-yl)methyl hydrogen phosphate;triethylammonium BDBM61258 cid_16396156 MLS001306424 [5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate;triethylazanium
(R)-2-amino-3-(4-octylphenylamino)-3-oxopropyl dihydrogen phosphate CHEMBL227371 BDBM50198840
2-formylphenoxyphosphonic acid BDBM14680 2-formylphenyl dihydrogen phosphate RU78262 CHEMBL286678 Fragment 10
2-hydrazinyl-2-oxoethyl hydrogen phosphate BDBM50167772 Phosphoric acid monohydrazinocarbonylmethyl ester CHEMBL195520
5-Amino-1-(beta-D-ribofuranosyl)-4-nitropyrazole 5'-phosphate CHEMBL554752 BDBM50247824
5-Amino-1-(beta-D-ribofuranosyl)pyrazole-4-carboxylate 5'-phosphate BDBM50247823
5-chloro-2-{[3-(trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (26) BDBM150746
5-chloro-2-{[4-(trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (27) BDBM150747
BDBM50198833 CHEMBL228102 (R)-2-amino-3-(3-octylphenylamino)-3-oxopropyl dihydrogen phosphate
BDBM50247809 5-Amino-1-(beta-D-ribofuranosyl)imidazole-4-carboxaldehyde 5'-phosphate
BDBM50312665 Sodium 2-(2-Fluorophenyl)-6,7-methylenedioxyquinolin-4-yl hydrogen phosphate CHEMBL1088572
BDBM50343853 2-(2-(Benzylamino)-6-nitroquinazolin-4-ylamino)ethyl dihydrogen phosphate CHEMBL1774786
CHEMBL1765479 BDBM50342010 5'-phospho-2'-deoxyuridine-3'-pyrophosphate(P'->5')adenosine3'-phosphate
(S)-2-amino-4-(4-(heptyloxy)phenyl)-2-methylbutyl dihydrogen phosphate BDBM50313499 CHEMBL1084929
2-(2-Oxo-1,2-dihydrobenzo[cd]indole-6-sulfonamido)ethyldihydrogen phosphate BDBM50316579 CHEMBL1095991
2-amino-4,5-dihydronaphtho[1,2-d]thiazol-7-yl dihydrogen phosphate CHEMBL566049 BDBM50302494
2-amino-4,5-dihydronaphtho[1,2-d]thiazol-9-yl dihydrogen phosphate BDBM50302496 CHEMBL576125
2-amino-8H-indeno[1,2-d]thiazol-4-yl dihydrogen phosphate CHEMBL578134 BDBM50302493
2-amino-8H-indeno[1,2-d]thiazol-5-yl dihydrogen phosphate BDBM50302492 CHEMBL568771
2-amino-8H-indeno[1,2-d]thiazol-6-yl dihydrogen phosphate BDBM50302491 CHEMBL566670
3-(4,5,6,7-Tetrabromo-2H-1,2,3-benzotriazol-2-yl)propyl dihydrogen phosphate BDBM50276680 CHEMBL459343
5-Amino-1-(beta-D-ribofuranosyl)-1,2,3-triazole-4-carboxylate 5'-phosphate BDBM50247825
BDBM150734 2‐[(4‐bromophenyl)carbamoyl]‐4‐chlorophenyl diethyl phosphate (14)
BDBM150736 2‐[(3‐bromophenyl)carbamoyl]‐4‐chlorophenyl diethyl phosphate (16)
BDBM50316580 6-(2-oxo-1,2-dihydrobenzo[cd]indole-6-sulfonamido)hexyldihydrogen phosphate CHEMBL1095867
CHEMBL1097477 4-(2-Oxo-1,2-dihydrobenzo[cd]indole-6-sulfonamido)-butyldihydrogen phosphate BDBM50316577
CHEMBL1097478 BDBM50316581 5-(2-Oxo-1,2-dihydrobenzo[cd]indole-6-sulfonamido)-pentyldihydrogen phosphate
CHEMBL455775 BDBM50292713 5'-phospho-2'-deoxyuridine 3-pyrophosphate (P'->5') adenosine 3'-phosphate
CHEMBL88487 Hexadecyl[2-(N-Methylmorpholinio)ethyl]phosphate BDBM50051809 (N-methylmorpholino)ethanol hexadecyl phosphonate
(3R,4S,5R)-4,5-dihydroxy-3-phosphonooxycyclohexene-1-carboxylic acid Shikimate-3-phosphate BDBM100283
(benzyloxy)(2-formylphenoxy)phosphinic acid PASBN BDBM14688 benzyl 2-formylphenyl hydrogen phosphate Fragment 18
1-(4-Methoxyphenylsulfonamido)-3-morpholinopropan-2-yl dihydrogen phosphate HEA derivative, 7a BDBM50248217 CHEMBL473552
1D-myo-inositol 1,3,4,5-tetrakis(dihydrogen phosphate) CHEMBL23552 BDBM50075651 1D-myo-inositol 1,3,4,5-tetrakisphosphate
1D-myo-inositol 1,4,5,6-tetrakisphosphate BDBM50075649 CHEMBL282059 1D-myo-inositol 1,4,5,6-tetrakis(dihydrogen phosphate)
1D-myo-inositol 4,5-bisphosphate BDBM50284584 CHEMBL21825 1D-myo-inositol 4,5-bis(dihydrogen phosphate)
2-(6-Nitro-2-(4-phenoxyphenylamino)quinazolin-4-ylamino)ethyl dihydrogen phosphate BDBM50343852 CHEMBL1774785
3-(2-Oxo-1,2-dihydrobenzo[cd]indole-6-sulfonamido)propyl dihydrogen phosphate BDBM50316578 CHEMBL1097476
4‐bromo‐2‐[(3‐fluorophenyl)carbamoyl]phenyl diethyl phosphate (1) BDBM150721
4‐bromo‐2‐[(4‐bromophenyl)carbamoyl]phenyl diethyl phosphate (8) BDBM150728
4‐bromo‐2‐[(4‐chlorophenyl)carbamoyl]phenyl diethyl phosphate (3) BDBM150723
4‐chloro‐2‐[(3‐chlorophenyl)carbamoyl]phenyl diethyl phosphate (10) BDBM150730
4‐chloro‐2‐[(3‐fluorophenyl)carbamoyl]phenyl diethyl phosphate (12) BDBM150732
4‐chloro‐2‐[(4‐chlorophenyl)carbamoyl]phenyl diethyl phosphate (11) BDBM150731
BDBM150722 4‐bromo‐2‐[(3‐chlorophenyl)carbamoyl]phenyl diethyl phosphate (2)
BDBM150724 4‐bromo‐2‐[(3,4‐dichlorophenyl)carbamoyl]phenyl diethyl phosphate (4)
BDBM150727 4‐bromo‐2‐[(3‐bromophenyl)carbamoyl]phenyl diethyl phosphate (7)
BDBM150729 4‐bromo‐2‐[(4‐fluorophenyl)carbamoyl]phenyl diethyl phosphate (9)
BDBM150733 4‐chloro‐2‐[(4‐fluorophenyl)carbamoyl]phenyl diethyl phosphate (13)
BDBM150735 4‐chloro‐2‐[(3,4‐dichlorophenyl)carbamoyl]phenyl diethyl phosphate (15)
BDBM50287679 CHEMBL61555 Sodium; benzyl 3,4,5-trihydroxy-6-methyltetrahydro-2H-thiopyran-2-yl hydrogen phosphate
BDBM50315803 2-amino-4-(2-fluoro-4-pentylphenyl)-2-(hydroxymethyl)butyl dihydrogen phosphate CHEMBL1095889
BDBM50315805 2-amino-4-(4'-butoxybiphenyl-4-yl)-2-(hydroxymethyl)butyl dihydrogen phosphate CHEMBL1096211
HEA derivative, 7b BDBM50248218 CHEMBL473752 1-(4-Fluorophenylsulfonamido)-3-morpholinopropan-2-yl dihydrogen phosphate
4-THIOURIDINE-5'-MONOPHOSPHATE 4-THIOURIDINE-5'-PHOSPHATE BDBM50341895 ((2R,3S,4R,5R)-3,4-dihydroxy-5-(2-oxo-4-thioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl dihydrogen phosphate CHEMBL1230331
dihydrogen phosphate;2-[2-(4-fluorophenyl)sulfanylethyl]pyridin-1-ium BDBM45710 2-[2-(4-Fluoro-phenylsulfanyl)-ethyl]-pyridinium dihydrogen phosphate;2-[2-[(4-fluorophenyl)thio]ethyl]pyridin-1-ium cid_16682387 MLS000767112 SMR000429527
O-phosphoryl-4-hydroxy-N,N-dimethyltryptamine 4-phosphoryloxy-N,N-dimethyltryptamine US11597738, Example 4 BDBM50171269 Indocybin psilocybin 3-[2-(dimethylamino)ethyl]-1H-indol-4-yl dihydrogen phosphate psilocin phosphate ester Psilocybine CHEMBL194378
(2S)-2-amino-3-(phosphonooxy)propanoic acid BDBM17664 CHEMBL284377 L-serine O-phosphate L-SOP
4‐chloro‐2‐{[3‐ (trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (17) BDBM150737
BDBM150725 4‐bromo‐2‐{[3‐ (trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (5)
BDBM150726 4‐bromo‐2‐{[4‐ (trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (6)
BDBM150738 4‐chloro‐2‐{[4‐ (trifluoromethyl)phenyl]carbamoyl}phenyl diethyl phosphate (18)
BDBM50110800 P,P'-Bis {[4-(guanine-9-yl)cyclopent-2-enyl]oxy-methylphosphonyl}phosphate CHEMBL23886
CHEMBL277903 P,P'-Bis {[4-(adenine-9-yl)cyclopent-2-enyl]oxy-methylphosphonyl}phosphate BDBM50110798
CHEMBL475495 (S)-2-amino-2-methyl-3-(4-(octyloxy)phenylamino)-3-oxopropyl dihydrogen phosphate BDBM50277148
PAS219 (cyclohexylmethoxy)(2-formylphenoxy)phosphinic acid BDBM14685 CHEMBL24811 Fragment 15 cyclohexylmethyl 2-formylphenyl hydrogen phosphate
MLS001306420 SMR000718790 [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate;hydrate cid_6419976 BDBM70140 [(2R,3S,4R,5R)-5-adenin-9-yl-3,4-dihydroxy-tetrahydrofuran-2-yl]methyl dihydrogen phosphate;hydrate [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl dihydrogen phosphate;hydrate [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-2-oxolanyl]methyl dihydrogen phosphate;hydrate
(1R,3S,4R,6R)-4,5,6-trihydroxy-2-methyl-2,3-bis(phosphonooxy)cyclohexyl dihydrogen phosphate BDBM50075648 CHEMBL281132
(R)-2-amino-2-((R)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propyl dihydrogen phosphate BDBM50314403 CHEMBL1089004
(S)-2-amino-2-((R)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propyl dihydrogen phosphate BDBM50314404 CHEMBL1089005
(S)-2-amino-2-((S)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propyl dihydrogen phosphate CHEMBL1090758 BDBM50314405
(S)-2-amino-3-(4-(4-cyclohexylbutoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate BDBM50249153 CHEMBL473562
1,2,3,4-tetrahydroquinolin-8-yl dihydrogen phosphate (1,2,3,4-tetrahydroquinolin-8-yloxy)phosphonic acid Fragment 12 RU79073 BDBM14682
BDBM14681 RU78299 CHEMBL421138 Fragment 11 2,6-diformyl-4-methylphenoxyphosphonic acid 2,6-diformyl-4-methylphenyl dihydrogen phosphate
BDBM50314402 (R)-2-amino-2-((S)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propyl dihydrogen phosphate CHEMBL1093686
BDBM50315804 2-amino-4-(3-chloro-4'-pentylbiphenyl-4-yl)-2-(hydroxymethyl)butyl dihydrogen phosphate CHEMBL1096210
CHEMBL195976 Phosphoric acid mono-(N-hydroxycarbamimidoylmethyl) ester BDBM50167775 (Z)-2-amino-2-(hydroxyimino)ethyl hydrogen phosphate
CHEMBL25186 RU78300 2-formyl-6-methoxyphenoxyphosphonic acid 2-formyl-6-methoxyphenyl dihydrogen phosphate BDBM14679 Fragment 9
CHEMBL611046 BDBM50304359 (2R,3S,5S,6S)-6-(hydrogen phosphonato)-3,5-dihydroxycyclohexane-1,2,4-triyl tris(hydrogen phosphate)
US10233190, Example 1434 BDBM370515 sodium 1-cyclohexyl-2-(5H-imidazo[5,1- a]isoindol-5-yl)ethyl phosphate
US11046646, Example 14 BDBM506618 (3-amino-1-(4-octylbenzyl)-2-oxopyrrolidin-3-yl)methyl dihydrogen phosphate
6-hydroxy-UMP 1-beta-D-ribofuranosyl(3H)pyrimidine-2,4,6-trione 5'-monophosphate 6-HYDROXYURIDINE-5'-PHOSPHATE 3,4-dihydroxy-5-(6-oxido-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl)methyl phosphate BDBM50199178 CHEMBL383923
(2R,3R,4S)-2,3,4-trihydroxy-5-(hydroxyamino)-5-oxopentyl phosphate BDBM50342610 5-PHOSPHO-D-ARABINOHYDROXAMIC ACID CHEMBL1235136
(R)-2-amino-2-(4-(4-(octyloxy)phenyl)-1H-imidazol-2-yl)propyl dihydrogen phosphate BDBM50277186 CHEMBL474689
(S)-2-amino-3-(2-fluoro-4-(octyloxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate CHEMBL474688 BDBM50277185
2,3,4-trihydroxy-5-oxohexane-1,6-diyl bis(dihydrogen phosphate) BDBM50330437 1,6-di-O-phosphono-D-fructose CHEMBL1235112
2-[hydroxy(4-hydroxybutyl)amino]-2-oxoethyl dihydrogen phosphate BDBM50330439 CHEMBL1236227 N-(4-hydroxybutyl)-phospho-glycolohydroxamic acid
2-amino-3-hydroxy-2-((R)-6-octyl-1,2,3,4-tetrahydronaphthalen-2-yl)propyl dihydrogen phosphate CHEMBL1091103 BDBM50314401
BDBM50080321 ZMP [5-(5-amino-4-carbamoyl-1H-imidazol-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate
BDBM50249114 CHEMBL473156 (S)-2-amino-2-methyl-3-oxo-3-(4-(5-phenylpentyloxy)phenylamino)propyl dihydrogen phosphate
BDBM50249154 CHEMBL473563 (S)-2-amino-3-(4-(biphenyl-4-ylmethoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate
BDBM50277149 CHEMBL515921 (S)-2-amino-3-(3-fluoro-4-(octyloxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate
CHEMBL1089558 BDBM50315807 2-amino-4-(4'-butoxy-2',5'-difluorobiphenyl-4-yl)-2-(hydroxymethyl)butyl dihydrogen phosphate
CHEMBL1683319 5-O-phosphate-1-N-(4'-(4''-methoxyhenyl))-[1',2',3']-triazol-beta-D-ribofuranoside BDBM50338502
CHEMBL514302 BDBM50249113 (S)-2-amino-2-methyl-3-oxo-3-(4-(4-phenylbutoxy)phenylamino)propyl dihydrogen phosphate
CHEMBL515603 (S)-2-amino-3-(4-(biphenyl-4-yloxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate BDBM50249238
[(2-cyanoquinolin-8-yl)oxy]phosphonic acid RU79072 Fragment 14 BDBM14684 2-cyanoquinolin-8-yl dihydrogen phosphate
(3-amino-1-(4-(1-(phenethoxyimino)ethyl)benzoyl)pyrrolidin-3-yl)methyl Dihydrogen Phosphate US11059784, Example 55 BDBM511446
(S)-2-amino-3-(4-(4-(4-methoxyphenyl)butoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate CHEMBL516035 BDBM50249151
BDBM25105 [3-(2,4-diamino-6-{3-[(diethoxyphosphoryl)oxy]phenyl}pteridin-7-yl)phenyl] diethyl phosphate diaminopteridine derivative, 28
BDBM50199187 CHEMBL217370 6-propyl-3-beta-D-ribofuranosyl(1H)pyrimidine-2,4-dione 2',3'-(cyclic phosphate) 5'-triphosphate
BDBM50249294 CHEMBL470511 (R)-2-amino-2-(4-(4-(5-phenylpentyloxy)phenyl)-1H-imidazol-2-yl)propyl dihydrogen phosphate
BDBM50272891 (4-(4-(trifluoromethyl)phenylamino)-7-(3-(trifluoromethyl)pyridin-2-yl)quinazolin-2-yl)methyl dihydrogen phosphate CHEMBL456982
CHEMBL179992 CHEMBL182645 BDBM50150613 [(1R,4S,7aR)-1-(1,5-dimethylhexyl)-4,7a-dimethyloctahydro-1H-inden-4-yl]methyl dihydrogen phosphate
CHEMBL515917 (R)-2-amino-2-(4-(4-(4-phenylbutoxy)phenyl)-1H-imidazol-2-yl)propyl dihydrogen phosphate BDBM50249293
FTY720-phosphate, rac-2 {2-amino-3-hydroxy-2-[2-(4-octylphenyl)ethyl]propoxy}phosphonic acid BDBM23163 CHEMBL114606
Fragment compound, 1 4-trifluoromethylcoumarin phosphate {[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]oxy}phosphonic acid BDBM22598
US9707205, 19 BDBM255780 4-(4-(3-(3-(2,2,2-trifluoroacetyl)-1H-indol-1-yl)propyl)phenyl)butyl-dihydrogen phosphate
((4R,5R)-5-(hydroxycarbamoyl)-2,2-dimethyl-1,3-dioxolan-4-yl)methyl dihydrogen phosphate Phosphoric acid mono-((R)-(R)-5-hydroxycarbamoyl-2,2-dimethyl-[1,3]dioxolan-4-ylmethyl) ester (5-(hydroxycarbamoyl)-2,2-dimethyl-1,3-dioxolan-4-yl)methyl dihydrogen phosphate CHEMBL324609 BDBM50148780
hexadecyl 2-(trimethylammonio)ethyl phosphate [2-(Hexadecyloxy-hydroxy-phosphoryloxy)-ethyl]-trimethyl-ammonium hexadecylphosphocholine hexadecyl 2-(trimethyl-lambda~5~-azanyl)ethyl hydrogen phosphate hexadecylphosphocholine, miltefosine MILTEFOSINE CHEMBL125 hexadecyloxy-2-trimethylammonioethylphosphorate 2-(((Hexadecyloxy)hydroxyphosphinyl)oxy)-N,N,N-trimethylethanaminium hydroxide BDBM50034220 n-hexadecylphosphocholine
CYTIDINE-5'-MONOPHOSPHATE Phosphoric acid mono-[5-(4-amino-2-oxo-2H-pyrimidin-1-yl)-3,4-dihydroxy-tetrahydro-furan-2-ylmethyl] ester CHEMBL307679 cytidine 5'-phosphate ((2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl dihydrogen phosphate BDBM50310540 ((2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1(2H)-yl)-3,4-dihydroxy-tetrahydrofuran-2-yl)methyl dihydrogen phosphate
((1R,3R)-1-amino-3-(4-(1-((cyclopropylmethoxy)imino)ethyl)phenyl)cyclopentyl)methyl Dihydrogen Phosphate BDBM511429 US11059784, Example 38
(4-chlorophenyl)(dimethoxyphosphoryl)methyl dimethyl phosphate BDBM50240444 [(4-Chloro-phenyl)-(dimethoxy-phosphoryloxy)-methyl]-phosphonic acid dimethyl ester MIFOBATE CHEMBL284768
(S)-2-amino-3-(4-(2-(biphenyl-3-yl)ethoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate BDBM50249262 CHEMBL473237
BDBM50249263 (S)-2-amino-3-(4-(3-(biphenyl-4-yl)propoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate CHEMBL473238
BDBM50249266 (R)-2-amino-2-(4-(4-(biphenyl-4-ylmethoxy)phenyl)-1H-imidazol-2-yl)propyl dihydrogen phosphate CHEMBL514170
CHEMBL1090796 (S)-2-amino-3-(4-(3-(biphenyl-4-yl)propanoyl)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate BDBM50315550
CHEMBL475253 (S)-2-amino-3-(4-(4-(biphenyl-4-yl)butoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate BDBM50249264
CHEMBL475405 BDBM50249240 (S)-2-amino-3-(4-(2-(biphenyl-4-yl)ethoxy)phenylamino)-2-methyl-3-oxopropyl dihydrogen phosphate
CHEMBL514189 (S)-2-amino-2-methyl-3-(4-(2-(naphthalen-1-yl)ethoxy)phenylamino)-3-oxopropyl dihydrogen phosphate BDBM50249239
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- ChEMBL_556521 (CHEMBL956516) Inhibition of rabbit muscle glycogen phosphorylase assessed as release of phosphate from glucose 1 phosphate
- ChEMBL_1558648 (CHEMBL3771525) Inhibition of rabbit muscle glycogen phosphorylase-a assessed as formation of inorganic phosphate from glucose-1-phosphate by colorimetry
- ChEMBL_595113 (CHEMBL1050600) Inhibition of rabbit muscle glycogen phosphorylase A assessed as release of phosphate from glucose-1-phosphate after 25 mins
- ChEMBL_620083 (CHEMBL1108551) Inhibition of rabbit muscle glycogen phosphorylase assessed as release of phosphate from glucose-1-phosphate after 25 mins
- ChEMBL_762684 (CHEMBL1817502) Inhibition of rabbit muscle glycogen phosphorylase 1a assessed as release of phosphate from glucose-1- phosphate after 20 mins
- ChEMBL_762685 (CHEMBL1817503) Inhibition of human liver glycogen phosphorylase 1a assessed as release of phosphate from glucose-1- phosphate after 20 mins
- ChEMBL_1360672 (CHEMBL3282830) Non-competitive inhibition of pyridoxine phosphate oxidase (unknown origin) assessed as oxidation of pyridoxol 5'-phosphate by Lineweaver-Burk plot analysis
- ChEMBL_1460187 (CHEMBL3369412) Inhibition of rabbit muscle glycogen phosphorylase a assessed as inhibition of release of phosphate from glucose-1-phosphate after 30 mins by spectrophotometry
- ChEMBL_1630551 (CHEMBL3873257) Inhibition of rabbit muscle glycogen phosphorylase-b assessed as release of inorganic phosphate from glucose-1-phosphate in presence of AMP and glycogen
- ChEMBL_99040 (CHEMBL712593) In vitro inhibitory activity against recombinant human liver glycogen phosphorylase a (rHLGPa) catalyzed release of phosphate from glucose-1-phosphate.
- Inhibition Activity Assay Human liver glycogen phsphorylase (HLGP) activity was measured in the direction of glycogen synthesis by the release of phosphate from glucose-1-phosphate.
- ChEMBL_447975 (CHEMBL898227) Inhibition of glucose 6 phosphate translocase 1
- ChEMBL_664344 (CHEMBL1259493) Inhibition of human recombinant renin in phosphate buffer
- ChEMBL_743385 (CHEMBL1767684) Inhibition of mTOR by radiometric phosphate incorporation assay
- ChEMBL_1733215 (CHEMBL4148751) Inhibition of rabbit muscle glycogen phosphorylase-a assessed as release of inorganic phosphate using glucose-1-phosphate as substrate in presence of glycogen by spectrophotometric method
- ChEMBL_1733216 (CHEMBL4148752) Inhibition of human liver glycogen phosphorylase-a assessed as release of inorganic phosphate using glucose-1-phosphate as substrate in presence of glycogen by spectrophotometric method
- ChEMBL_1670145 (CHEMBL4020033) Competitive inhibition of human liver glycogen phosphorylase-a assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen preincubated for 15 mins with AMP and glycogen followed by glucose-1-phosphate addition
- ChEMBL_1619029 (CHEMBL3861198) Inhibition of rabbit skeletal muscle glycogen phosphorylase b assessed as reduction in inorganic phosphate release using glucose-1-phosphate as substrate at pH 6.8
- ChEMBL_753234 (CHEMBL1799389) Inhibition of rabbit muscle glycogen phosphorylase A assessed as release of phosphate from glucose-1-phosphate after 25 mins by microplate reader based method
- ChEMBL_797827 (CHEMBL1942413) Inhibition of rabbit muscle glycogen phosphorylase a assessed as release of phosphate from glucose-1-phosphate after 25 mins using malachite green staining
- ChEMBL_36028 (CHEMBL646429) Inhibitory activity against Arabinose-5-phosphate isomerase
- ChEMBL_447602 (CHEMBL896616) Inhibition of human PNP in presence of 1 mM phosphate
- ChEMBL_447603 (CHEMBL896617) Inhibition of human PNP in presence of 0.025 mM phosphate
- ChEMBL_447604 (CHEMBL896618) Inhibition of calf PNP in presence of 1 mM phosphate
- ChEMBL_447605 (CHEMBL896619) Inhibition of calf PNP in presence of 0.025 mM phosphate
- ChEMBL_72881 (CHEMBL684049) TInhibition of glyceraldehyde-3-phosphate dehydrogenase in Leishmania mexicana
- ChEMBL_72891 (CHEMBL684058) Inhibition of glyceraldehyde-3-phosphate dehydrogenase in Trypanosoma brucei
- ChEMBL_72903 (CHEMBL684097) Inhibition of Glyceraldehyde-3-phosphate dehydrogenase in human erythrocytes.
- ChEMBL_765397 (CHEMBL1828245) Inhibition of PTP1B using p-nitrophenyl phosphate as substrate
- ChEMBL_775058 (CHEMBL1912253) Noncompetitive inhibition of GFAT in presence of fructose-6-phosphate
- ChEMBL_882108 (CHEMBL2214496) Inhibition of TCPTP by para-nitrophenyl phosphate release assay
- ChEMBL_882109 (CHEMBL2214497) Inhibition of PTP1B by para-nitrophenyl phosphate release assay
- ChEMBL_1619018 (CHEMBL3861187) Inhibition of rabbit skeletal muscle glycogen phosphorylase b assessed as inorganic phosphate release using glucose-1-phosphate as substrate by double-reciprocal plot analysis
- ChEMBL_1733214 (CHEMBL4148750) Inhibition of rabbit muscle glycogen phosphorylase-b assessed as release of inorganic phosphate using glucose-1-phosphate as substrate in presence of AMP and glycogen by spectrophotometric method
- Fluorescent Coupled Assay The binding affinities to FtsZ were determined by a fluorescent coupled assay for phosphate relase. In this GTPase studies, phosphate liberated during GTP hydrolysis is a substrate of purine nucleoside phosphorylase, which converts 7-methylguanosine to 7-methylguanin and ribose-1-phosphate, resulting in a decrease of fluorescence.
- ChEMBL_2106081 (CHEMBL4814756) Inhibition of Fam20C (unknown origin) assessed as reduction in inorganic phosphate release in presence of ATP measured by Malachite green phosphate staining based micro plate reader assay
- ChEMBL_764140 (CHEMBL1821351) Competitive inhibition of rabbit muscle glycogen phosphorylase b assessed as inorganic phosphate release using alphaD glucose-1-phosphate as substrate at pH 6.8 by spectrophotometric analysis
- ChEMBL_1455461 (CHEMBL3363893) Stimulation of ATPase activity of human MRP2 assessed as inorganic phosphate
- ChEMBL_1455462 (CHEMBL3363894) Inhibition of ATPase activity of human MRP2 assessed as inorganic phosphate
- ChEMBL_1455463 (CHEMBL3363895) Stimulation of ATPase activity of rat Mrp2 assessed as inorganic phosphate
- ChEMBL_1455464 (CHEMBL3363896) Inhibition of ATPase activity of rat Mrp2 assessed as inorganic phosphate
- ChEMBL_302548 (CHEMBL828250) Binding affinity against rabbit muscle triose-phosphate isomerase
- ChEMBL_305228 (CHEMBL831647) Inhibitory concentration against rabbit muscle triose-phosphate isomerase
- ChEMBL_555945 (CHEMBL959129) Activity at human GnRH receptor by inositol phosphate functional assay
- ChEMBL_775079 (CHEMBL1912274) Inhibition of GFAT using N-acetylglucosamine 6-phosphate as substrate
- ChEMBL_835427 (CHEMBL2072133) Agonist activity at mouse GPR142 assessed as inositol phosphate accumulation
- ChEMBL_882103 (CHEMBL2214491) Competitive inhibition of PTP1B by para-nitrophenyl phosphate release assay
- Inhibition Assay Inhibition assay using glucose-1-phosphate thymidylyltransferase (RmlA).
- Inhibition Assay Inhibition assay using sphingosine-1-phosphate receptor 3.
- ChEMBL_1649508 (CHEMBL3998642) Inhibition of rat muscle glycogen phosphorylase A assessed as release of inorganic phosphate from glucose-1- phosphate in presence of glycogen after 30 mins by malachite green based assay
- ChEMBL_907777 (CHEMBL3066369) Inhibition of Escherichia coli imidazoleglycerol phosphate dehydratase using imidazole glycerol phosphate as substrate incubated for 30 min prior to substrate addition measured for 16 min by spectrophotometric analysis
- ChEMBL_1878663 (CHEMBL4380057) Inhibition of human recombinant CYP1B1 using 7-ethoxyresorufin as substrate in presence of glucose-6-phosphate, glucose-6-phosphate dehydrogenase and NADPH-generating system incubated for 35 mins by fluorometry
- ChEMBL_1878664 (CHEMBL4380058) Inhibition of human recombinant CYP1A1 using 7-ethoxyresorufin as substrate in presence of glucose-6-phosphate, glucose-6-phosphate dehydrogenase and NADPH-generating system incubated for 15 mins by fluorometry
- ChEMBL_1878665 (CHEMBL4380059) Inhibition of human recombinant CYP1A2 using 7-ethoxyresorufin as substrate in presence of glucose-6-phosphate, glucose-6-phosphate dehydrogenase and NADPH-generating system incubated for 50 mins by fluorometry
- ChEMBL_506139 (CHEMBL937902) Inhibition of Mycobacterium tuberculosis DXR assessed as NADPH-dependent conversion of 1-deoxy-D-xylulose 5-phosphate to 2-C-methyl-D-erythritol 4-phosphate by spectrophotometric method
- ChEBML_158626 Inhibition of human prostatic acid phosphatase was determined as inhibition of the hydrolysis of tyrosine phosphate
- ChEBML_52541 Inhibition of 2-C-methyl-d-erythritol-4-phosphate cytidyltransferase (YgbP)
- ChEBML_69909 Inhibitory concentration against Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEBML_71454 Inhibition of gonadotropin-releasing hormone receptor-stimulated [3H]inositol phosphate hydrolysis
- ChEBML_72905 Compound was tested for the inhibition of Leishmania mexicana GAPDH(glyceraldehyde-3-phosphate dehydrogenase)
- ChEMBL_1581173 (CHEMBL3810646) Inhibition of recombinant human PTP1B assessed as hydrolysis of p-nitrophenyl phosphate
- ChEMBL_162205 (CHEMBL767214) Binding affinity against phosphate binding site of Purine nucleoside Phosphorylase
- ChEMBL_303122 (CHEMBL829631) Inhibitory activity against Escherichia coli deoxyxylulose 5-phosphate reductoisomerase
- ChEMBL_466588 (CHEMBL930238) Displacement of pentamannosyl phosphate bovine serum albumin from M6P/IGF2R
- ChEMBL_499713 (CHEMBL980648) Displacement of [33P]sphingosine-1-phosphate from human S1P1 receptor
- ChEMBL_499714 (CHEMBL980649) Displacement of [33P]sphingosine-1-phosphate from human S1P3 receptor
- ChEMBL_499715 (CHEMBL980650) Displacement of [33P]sphingosine-1-phosphate from human S1P4 receptor
- ChEMBL_499716 (CHEMBL980651) Displacement of [33P]sphingosine-1-phosphate from human S1P5 receptor
- ChEMBL_519984 (CHEMBL951813) Inhibition of sphingosine-1-phosphate lyase in mouse spleen by scintillation counting
- ChEMBL_540418 (CHEMBL1029035) Displacement of [33P]sphingosine-1-phosphate from human S1P1 receptor
- ChEMBL_540419 (CHEMBL1029036) Displacement of [33P]sphingosine-1-phosphate from human S1P3 receptor
- ChEMBL_540420 (CHEMBL1029037) Displacement of [33P]sphingosine-1-phosphate from human S1P4 receptor
- ChEMBL_540421 (CHEMBL1029038) Displacement of [33P]sphingosine-1-phosphate from human S1P5 receptor
- ChEMBL_555946 (CHEMBL959130) Activity at rhesus monkey GnRH receptor by inositol phosphate functional assay
- ChEMBL_606269 (CHEMBL1069959) Agonist activity against 5HT2B receptor assessed as inositol phosphate accumulation
- ChEMBL_70744 (CHEMBL681455) Inhibition of rat brain farnesyltransferase in 30 mM potassium phosphate buffer
- ChEMBL_72894 (CHEMBL684061) Inhibitory concentration of compound against glyceraldehyde-3-phosphate dehydrogenase was determined
- ChEMBL_72896 (CHEMBL684063) Inhibitory activity was measured for Glyceraldehyde-3-phosphate dehydrogenase in Trypanosoma brucei
- ChEMBL_72898 (CHEMBL684065) Inhibitory activity was measured for Glyceraldehyde-3-phosphate dehydrogenase in Trypanosoma cruzi.
- ChEMBL_72900 (CHEMBL684215) Inhibitory activity was measured for Glyceraldehyde-3-phosphate dehydrogenase in human(erythrocytes)
- ChEMBL_741887 (CHEMBL1768448) Inhibition of Itk after 40 mins by radiometric phosphate incorporation assay
- ChEMBL_743384 (CHEMBL1767683) Inhibition of DNAPK after 2 hrs by radiometric phosphate incorporation assay
- ChEMBL_986855 (CHEMBL2438603) Inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate as substrate
- ChEMBL_1632373 (CHEMBL3875079) Inhibition of PI3Kbeta in HUVEC assessed as reduction in sphingosine-1-phosphate stimulated akt phosphorylation at Ser473 residue preincubated for 15 mins followed by sphingosine-1-phosphate stimulation for 5 mins by HTRF assay
- ChEMBL_604866 (CHEMBL1065975) Inhibition of Escherichia coli type I phosphomannose isomerase assessed as D-mannose 6-phosphate to D-fructose 6-phosphate isomerization at pH 7.1 by PGI/G6PDH coupled enzyme spectrophotometric assay
- ChEMBL_604867 (CHEMBL1065976) Inhibition of Saccharomyces cerevisiae type I phosphomannose isomerase assessed as D-mannose 6-phosphate to D-fructose 6-phosphate isomerization at pH 7.1 by PGI/G6PDH coupled enzyme spectrophotometric assay
- ChEBML_153319 In vitro inhibition of purine nucleoside phosphorylase from calf spleen in 1 mM phosphate
- ChEBML_65103 Competitive inhibitory activity with respect to EPSP (5-enolpyruvyl-shikimate- 3-phosphate) synthase
- ChEBML_72895 Inhibitory concentration against glyceraldehyde-3-phosphate dehydrogenase was determined as log 1/IC50
- ChEBML_90659 Inhibitory concentration against imidazole glycerol phosphate dehydratase (IGPD) from Cryptococcus neoformans
- ChEMBL_1286129 (CHEMBL3106986) Competitive inhibition of PFKFB3 (unknown origin) using fructose 6-phosphate as substrate
- ChEMBL_1455848 (CHEMBL3362302) Inhibition of rat DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically
- ChEMBL_1455849 (CHEMBL3362303) Inhibition of human DNPH1 assessed as 2-deoxyribose 5-phosphate production by spectrophotometrically
- ChEMBL_1467560 (CHEMBL3411611) Competitive inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate as substrate
- ChEMBL_147577 (CHEMBL750155) Accumulation of inositol phosphate in 1321N1 astrocytoma cells expressing human P2Y1 purinoceptor
- ChEMBL_1500296 (CHEMBL3588720) Inverse agonist activity at rat ghrelin receptor by inositol phosphate turnover assay
- ChEMBL_1506090 (CHEMBL3595956) Inhibition of human placental alkaline phosphatase using p-nitrophenyl phosphate substrate
- ChEMBL_153320 (CHEMBL760056) Inhibition of purine nucleoside phosphorylase from calf spleen in 50 mM phosphate
- ChEMBL_162032 (CHEMBL770312) Inhibition of calf spleen PNP (purine nucleoside phosphorylase) in 1 mM phosphate
- ChEMBL_162033 (CHEMBL766673) Inhibition of calf spleen PNP (purine nucleoside phosphorylase) in 50 mM phosphate
- ChEMBL_162190 (CHEMBL766710) Inhibition of human erythrocytic PNP (purine nucleoside phosphorylase) in 1 mM phosphate
- ChEMBL_1888918 (CHEMBL4390595) Inhibition of Mycobacterium tuberculosis H37Ra PTPB using p-nitrophenyl phosphate as substrate
- ChEMBL_1990679 (CHEMBL4624414) Inhibition of HSP90 ATPase activity (unknown origin) malachite-green phosphate assay
- ChEMBL_2224918 (CHEMBL5138431) Inhibition of recombinant Trypanosoma cruzi TIM using glyceraldehyde 3-phosphate as substrate
- ChEMBL_306074 (CHEMBL874554) Inhibition of human GnRH receptor stimulated inositol phosphate accumulation in RBL cells
- ChEMBL_312533 (CHEMBL832576) Inhibition of PGD2-induced inositol phosphate formation at human Prostaglandin D2 receptor
- ChEMBL_399838 (CHEMBL910317) Inhibition of non-phosphorylated form of rabbit muscle glycogen phosphorylase in presence of phosphate
- ChEMBL_463125 (CHEMBL929037) Inhibition of human carbonic anhydrase 1 assessed as 4-nitrophenyl phosphate hydrolysis
- ChEMBL_463126 (CHEMBL929038) Inhibition of human carbonic anhydrase 2 assessed as 4-nitrophenyl phosphate hydrolysis
- ChEMBL_463127 (CHEMBL929039) Inhibition of mouse carbonic anhydrase 13 assessed as 4-nitrophenyl phosphate hydrolysis
- ChEMBL_504639 (CHEMBL993810) Inhibition of DNA polymerase beta lyase activity by deoxyribose phosphate excision assay
- ChEMBL_516599 (CHEMBL988686) Inhibition of fructose-1,6-bisphosphatase in mouse liver homogenates by colorimetric phosphate assay
- ChEMBL_566013 (CHEMBL964112) Inhibition of ATPase activity of SCV helicase assessed as phosphate release by colorimetric assay
- ChEMBL_598030 (CHEMBL1046299) Inhibition of Streptococcus pneumoniae SP-3 gyrB by enzyme coupled phosphate assay
- ChEMBL_69909 (CHEMBL682259) Inhibitory concentration against Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69910 (CHEMBL682260) Inhibitory concentration against Trypanosoma brucei glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69911 (CHEMBL682261) Inhibitory concentration against Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69912 (CHEMBL682262) Inhibitory concentration against Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69913 (CHEMBL682263) Inhibitory concentration against Trypanosoma mexicana glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69915 (CHEMBL682265) Inhibitory concentration against rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_69916 (CHEMBL877826) Inhibitory concentration against rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
- ChEMBL_71454 (CHEMBL685983) Inhibition of gonadotropin-releasing hormone receptor-stimulated [3H]inositol phosphate hydrolysis
- ChEMBL_72770 (CHEMBL681383) Inhibitory activity measured for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Leishmania. mexicana
- ChEMBL_72772 (CHEMBL681385) Inhibitory activity was measured for Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in Leishmania. mexicana.
- ChEMBL_72905 (CHEMBL874134) Compound was tested for the inhibition of Leishmania mexicana GAPDH(glyceraldehyde-3-phosphate dehydrogenase)
- ChEMBL_741888 (CHEMBL1768449) Inhibition of c-Kit after 45 mins by radiometric phosphate incorporation assay
- ChEMBL_831739 (CHEMBL2065102) Inhibition of KHKC in human HepG2 cells assessed as level of fructose-1-phosphate
- ChEMBL_1670147 (CHEMBL4020035) Competitive inhibition of 6xHis-tagged human liver glycogen phosphorylase-a expressed in Escherichia coli BL21 Gold (DE3) assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen preincubated for 15 mins with AMP and glycogen followed by glucose-1-phosphate addition by Lineweaver-Burk plot method
- ChEMBL_1619019 (CHEMBL3861188) Inhibition of human recombinant liver glycogen phosphorylase A expressed in baculovirus infected Sf9 insect cells assessed as release of phosphate from glucose-1- phosphate in presence of 7.5 mM glucose by malachite green based assay
- ChEMBL_1630550 (CHEMBL3873256) Competitive inhibition of rabbit muscle glycogen phosphorylase-a assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen measured after 1 min by Dixon plot method
- ChEMBL_1924594 (CHEMBL4427550) Activation of human recombinant glucokinase using 5 mM glucose monitored over 5 mins in presence of NAD+ and glucose 6-phosphate 4% human serum albumin by glucose 6-phosphate dehydrogenase coupled assay
- Enzyme Inhibition Assay The activity of the compounds is determined by measuring the inhibitory effect of the compounds in the direction of glycogen synthesis, the conversion of glucose-1-phosphate into glycogen with the release of inorganic phosphate, which was monitored using microplate reader (BIO-RAD). The phosphate absorbance was measured at 655 nm. The IC50 values were estimated by fitting the inhibition data to a dose dependent curve using a logistic derivative equation.
- ChEBML_162111 Inhibitory potency for the protein tyrosine phosphatase (PTP 1B)-catalyzed hydrolysis of p-nitrophenol phosphate
- ChEBML_1685483 Inhibition of recombinant human PTP1B using p-nitrophenyl phosphate as substrate after 30 mins
- ChEBML_64958 Dissociation constant was calculated for Escherichia coli EPSP(5-enolpyruvyl-shikimate-3-phosphate) Synthase
- ChEBML_65094 The compound was tested for the inhibition of Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSP).
- ChEMBL_1449753 (CHEMBL3378176) Inhibition of rabbit skeletal muscle glycogen phosphorylase b assessed as inorganic phosphate release
- ChEMBL_1458320 (CHEMBL3369282) Inhibition of SHP2 (unknown origin) using p-nitrophenyl phosphate substrate by microplate spectrophotometry
- ChEMBL_1462478 (CHEMBL3398881) Inhibition of STEP (unknown origin) in absence of GSH by p-nitrophenyl phosphate hydrolysis assay
- ChEMBL_153319 (CHEMBL760055) In vitro inhibition of purine nucleoside phosphorylase from calf spleen in 1 mM phosphate
- ChEMBL_1548347 (CHEMBL3755949) Inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate by spectrophotometric analysis
- ChEMBL_1554435 (CHEMBL3767566) Competitive inhibition of rabbit muscle glycogen phosphorylase b in presence of glucose 1-phosphate
- ChEMBL_1586214 (CHEMBL3819929) Inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate as substrate by colorimetric method
- ChEMBL_1586215 (CHEMBL3819930) Inhibition of TCPTP (unknown origin) using p-nitrophenyl phosphate as substrate by colorimetric method
- ChEMBL_1752096 (CHEMBL4186856) Agonist activity at P2Y2 receptor (unknown origin) assessed as reduction in inositol phosphate accumulation
- ChEMBL_312848 (CHEMBL874927) Inhibition of U-46,619-induced inositol phosphate accumulation at human Thromboxane A2 receptor
- ChEMBL_322135 (CHEMBL885194) Effective concentration against US28-mediated inositol phosphate production in COS-7 cells
- ChEMBL_424478 (CHEMBL912519) Activity at human NK3 receptor assessed as stimulation of inositol phosphate generation in CHO cells
- ChEMBL_48586 (CHEMBL662466) Inhibition of inositol phosphate production induced by Cholecystokinin type B receptor (0.5 nM)
- ChEMBL_542431 (CHEMBL1011499) Inhibition of human G6PDH using glucose-6-phosphate as substrate by Lineweaver-Burke plot
- ChEMBL_64950 (CHEMBL675695) Tested for the inhibition against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase
- ChEMBL_72895 (CHEMBL684062) Inhibitory concentration against glyceraldehyde-3-phosphate dehydrogenase was determined as log 1/IC50
- ChEMBL_860252 (CHEMBL2169193) Agonist activity at mouse GPR142 expressed in HEK293 cells by inositol phosphate accumulation assay
- ChEMBL_90659 (CHEMBL700890) Inhibitory concentration against imidazole glycerol phosphate dehydratase (IGPD) from Cryptococcus neoformans
- ChEMBL_90660 (CHEMBL700891) Binding affinity imidazole glycerol phosphate dehydratase (IGPD) obtained from Cryptococcus neoformans
- Enzyme Assay The rate of carbamoyl phosphate synthesis was determined by use of a coupled enzyme assay with ornithine transcarbamoylase.
- ChEMBL_767067 (CHEMBL1826116) Inhibition of Mycobacterium tuberculosis DXR assessed as reduction of 1-deoxy-D-xylulose 5-phosphate into 2-C-methyl-D-erythritol-4-phosphate measured for every 5 secs upto 500 secs by spectrophotometry analysis
- ChEBML_153324 Inhibition of Purine nucleoside phosphorylase was evaluated against the enzyme from calf spleen in 50 mM phosphate
- ChEBML_153325 Inhibition of Purine nucleoside phosphorylase was evaluated against the enzyme from Human erythrocytic in 50 mM phosphate
- ChEBML_1651766 Inhibition of recombinant human ATX using bisP-nitrophenyl phosphate as substrate measured after 30 mins
- ChEBML_1717872 Inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate as substrate measured after 30 mins
- ChEBML_64947 Compound was tested for inhibitory activity against EPSP(5-enolpyruvylshikimate 3-phosphate) synthase in Escherichia coli.
- ChEBML_65093 Tested for the inhibition against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase versus phosphoenolpyruvate
- ChEBML_70678 KI for pyridoxal 5'-phosphate (PLP)-dependent Gamma-amino-N-butyrate transaminase at pH 7.4
- ChEMBL_1335408 (CHEMBL3239059) Inhibition of PTP1B (unknown origin) using nitrophenyl phosphate as substrate after 30 mins by spectrophotometry
- ChEMBL_1362995 (CHEMBL3292563) Agonist activity at human GPR40 expressed in mouse A9 cells by inositol phosphate accumulation assay
- ChEMBL_1367645 (CHEMBL3299296) Inhibition of CDK1 (unknown origin) assessed as inhibition of phosphate incorporation into substrate in presence of radiolabeled ATP
- ChEMBL_1367646 (CHEMBL3299297) Inhibition of CDK5 (unknown origin) assessed as inhibition of phosphate incorporation into substrate in presence of radiolabeled ATP
- ChEMBL_1367647 (CHEMBL3299298) Inhibition of DYRK1A (unknown origin) assessed as inhibition of phosphate incorporation into substrate in presence of radiolabeled ATP
- ChEMBL_1367652 (CHEMBL3299303) Inhibition of CLK1 (unknown origin) assessed as inhibition of phosphate incorporation into substrate in presence of radiolabeled ATP
- ChEMBL_1449543 (CHEMBL3375115) Inhibition of rabbit muscle glycogen phosphorylase b at pH 6.8 assessed as inorganic phosphate release
- ChEMBL_1461022 (CHEMBL3395080) Inhibition of PRL3 (unknown origin) using DiFMUP as fluorogenic phosphate substrate assessed as DiFMUP dephosphorylation
- ChEMBL_1461023 (CHEMBL3395081) Inhibition of PRL2 (unknown origin) using DiFMUP as fluorogenic phosphate substrate assessed as DiFMUP dephosphorylation
- ChEMBL_1461024 (CHEMBL3395082) Inhibition of PRL1 (unknown origin) using DiFMUP as fluorogenic phosphate substrate assessed as DiFMUP dephosphorylation
- ChEMBL_1476393 (CHEMBL3430023) Inhibition of recombinant human PTP1B using p-nitrophenyl phosphate as substrate after 40 mins
- ChEMBL_1497576 (CHEMBL3582959) Inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate as substrate after 30 mins
- ChEMBL_153321 (CHEMBL760057) Inhibition of purine nucleoside phosphorylase against the enzyme from calf spleen in 1 mM phosphate
- ChEMBL_1575591 (CHEMBL3803908) Inhibition of human sphingosine 1-phosphate lyase after 22 hrs by umbelliferone fluorescence analysis
- ChEMBL_1586209 (CHEMBL3819924) Inhibition of human TCPTP using p-nitrophenyl phosphate as substrate by micro plate reader method
- ChEMBL_1705037 (CHEMBL4056270) Inhibition of PTP1B (unknown origin) using p-nitrophenyl phosphate substrate incubated for 30 mins
- ChEMBL_1705038 (CHEMBL4056271) Inhibition of SHP2 (unknown origin) using p-nitrophenyl phosphate substrate incubated for 30 mins
- ChEMBL_1705039 (CHEMBL4056272) Inhibition of PTPLAR (unknown origin) using p-nitrophenyl phosphate substrate incubated for 30 mins
- ChEMBL_1705040 (CHEMBL4056273) Inhibition of TCPTP (unknown origin) using p-nitrophenyl phosphate substrate incubated for 30 mins
- ChEMBL_1746765 (CHEMBL4181275) Inhibition of SHP2 (unknown origin) using 3-o-methylfluorescein phosphate as substrate by fluorescence assay
- ChEMBL_1746770 (CHEMBL4181280) Inhibition of SHP1 (unknown origin) using 3-o-methylfluorescein phosphate as substrate by fluorescence assay
- ChEMBL_1844001 (CHEMBL4344428) Binding affinity to bovine beta-trypsin in phosphate/HEPES/Tricine/Tris buffer by ITC method
- ChEMBL_1844002 (CHEMBL4344429) Binding affinity to human alpha-thrombin in phosphate/HEPES/Tricine/Tris buffer by ITC method
- ChEMBL_1986994 (CHEMBL4620541) Inhibition of SHP2 (unknown origin) using 3-o-methylfluorescein phosphate as substrate by fluorescence assay
- ChEMBL_1986997 (CHEMBL4620544) Inhibition of SHP1 (unknown origin) using 3-o-methylfluorescein phosphate as substrate by fluorescence assay
- ChEMBL_2015157 (CHEMBL4668735) Inhibition of human CD73 using AMP as substrate by Malachite green phosphate reagent-based assay
- ChEMBL_208010 (CHEMBL816090) Inhibition of HSV-1 thymidine kinase, 1 uM [3H]thymidine and ATP as phosphate donor
- ChEMBL_208029 (CHEMBL811287) Inhibition of HSV-2 thymidine kinase, 2 uM [3H]thymidine and ATP as phosphate donor
- ChEMBL_303534 (CHEMBL839648) In vitro inhibitory concentration against Protein-tyrosine phosphatase 1B hydrolysis of p-nitrophenol phosphate
- ChEMBL_306715 (CHEMBL832300) Inhibitory concentration against human glutathione reductase in the absence of glucose-6-phosphate dehydrogenase (G6PDH)
- ChEMBL_306877 (CHEMBL828683) Inhibition of CCK-Induced inositol phosphate production in Cos-7 cells expressing human CCK1R
- ChEMBL_317580 (CHEMBL825244) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (I352A)
- ChEMBL_317584 (CHEMBL838082) Inhibition of CCK-induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (F107A)
- ChEMBL_317585 (CHEMBL825581) Inhibition of CCK-induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (I329A)
- ChEMBL_317586 (CHEMBL825582) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (I329F)
- ChEMBL_317587 (CHEMBL825583) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (I352A)
- ChEMBL_317588 (CHEMBL825584) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (M195L)
- ChEMBL_317589 (CHEMBL825585) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (R197M)
- ChEMBL_317590 (CHEMBL825586) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (R336M)
- ChEMBL_317591 (CHEMBL825587) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (S342A)
- ChEMBL_317592 (CHEMBL825588) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (S348A)
- ChEMBL_317593 (CHEMBL825589) Inhibition of CCK-Induced inositol phosphate in Cos-7 cells expressing mutant CCK1R (T117A)
- ChEMBL_382561 (CHEMBL869767) Inhibition of kinesin ATPase activity by enzyme-linked inorganic phosphate detection assay without preincubation
- ChEMBL_424431 (CHEMBL910710) Activity at human NK3 receptor expressed in CHO cells assessed as increase in inositol phosphate generation
- ChEMBL_430293 (CHEMBL913919) Displacement of [33P]sphingosine 1 phosphate from human S1P1 receptor expressed in CHO cells
- ChEMBL_430294 (CHEMBL913920) Displacement of [33P]sphingosine 1 phosphate from human S1P2 receptor expressed in CHO cells
- ChEMBL_430295 (CHEMBL913921) Displacement of [33P]sphingosine 1 phosphate from human S1P3 receptor expressed in CHO cells
- ChEMBL_430296 (CHEMBL913922) Displacement of [33P]sphingosine 1 phosphate from human S1P4 receptor expressed in CHO cells
- ChEMBL_430297 (CHEMBL913923) Displacement of [33P]sphingosine 1 phosphate from human S1P5 receptor expressed in CHO cells
- ChEMBL_542429 (CHEMBL1010666) Inhibition of Trypanosoma brucei G6PDH using glucose-6-phosphate as substrate by Lineweaver-Burke plot
- ChEMBL_581483 (CHEMBL1055727) Inhibition of ATPase activity of SARS coronavirus helicase assessed as phosphate release by malachite green assay
- ChEMBL_590794 (CHEMBL1037632) Agonist activity at human NK3 receptor expressed in cells assessed as accumulation of [3H]inositol phosphate
- ChEMBL_598343 (CHEMBL1041717) Inhibition of recombinant human PTP1B assessed as hydrolysis of p-nitrophenyl phosphate after 30 mins
- ChEMBL_602838 (CHEMBL1065282) Antagonist activity at human mGluR5 assessed as inhibition of quisqualate-induced intracellular inositol phosphate accumulation
- ChEMBL_63499 (CHEMBL676490) Ability to block the ET-1-induced hydrolysis of inositol phosphate in Endothelin A receptor of MMQ cells.
- ChEMBL_64958 (CHEMBL675701) Dissociation constant was calculated for Escherichia coli EPSP(5-enolpyruvyl-shikimate-3-phosphate) Synthase
- ChEMBL_64961 (CHEMBL676327) Tested for the dissociation constant against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase
- ChEMBL_64963 (CHEMBL676329) The compound was tested for the inhibition of Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSP).
- ChEMBL_65087 (CHEMBL673144) Binding affinity against Escherichia coli EPSP(5-enolpyruvyl-shikimate-3-phosphate) synthase
- ChEMBL_65094 (CHEMBL670571) The compound was tested for the inhibition of Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSP).
- ChEMBL_65099 (CHEMBL670576) Tested for the dissociation rate against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase
- ChEMBL_65101 (CHEMBL670739) Tested for the rate of association against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase
- ChEMBL_70746 (CHEMBL681606) Inhibition of rat brain farnesyltransferase in 5 mM potassium phosphate and 50 mM HEPES buffer
- ChEMBL_72880 (CHEMBL684048) Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of Leishmania mexicana
- ChEMBL_72889 (CHEMBL684056) Tested for the inhibitory activity against Glycosomal glyceraldehyd 3-phosphate dehydrogenase (gGAPDH) of Trypanosoma brucei
- ChEMBL_72890 (CHEMBL684057) Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of Trypanosoma brucei
- ChEMBL_72902 (CHEMBL684096) Tested for the inhibitory activity against Glycosomal glyceraldehyde 3-phosphate dehydrogenase (gGAPDH) of human erythrocyte
- ChEMBL_743338 (CHEMBL1767585) Inhibition of full length recombinant ATR after 24 hrs by radiometric phosphate incorporation assay
- ChEMBL_743383 (CHEMBL1767682) Inhibition of full length recombinant ATM after 24 hrs by radiometric phosphate incorporation assay
- ChEMBL_744737 (CHEMBL1772758) Agonist activity at human 5HT2C receptor expressed in HEK293 cells by inositol phosphate accumulation assay
- ChEMBL_744739 (CHEMBL1772760) Agonist activity at human 5HT2B receptor expressed in HEK293 cells by inositol phosphate accumulation assay
- ChEMBL_748961 (CHEMBL1781409) Inhibition of recombinant human PTP1B using p-nitrophenyl phosphate as substrate after 30 mins
- ChEMBL_753876 (CHEMBL1798138) Inhibition of Calmodulin-dependent PDE1 activity assessed as inorganic phosphate release after 30 min
- ChEMBL_802298 (CHEMBL1949456) Inhibition of rabbit muscle glycogen phosphorylase b assessed as inorganic phosphate release at pH 6.8
- ChEMBL_839558 (CHEMBL2090365) Agonist activity at P2Y6 receptor expressed in human 1321N1 cells assessed as inositol phosphate accumulation
- ChEMBL_856703 (CHEMBL2162374) Inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate after 30 mins
- ChEMBL_862643 (CHEMBL2174116) Inhibition of human glucose-6-phosphate dehydrogenase after 90 mins by resazurin/diaphorasecoupled assay
- ChEMBL_862644 (CHEMBL2174117) Inhibition of Plasmodium falciparum glucose-6-phosphate dehydrogenase after 45 mins by orthogonal assay
- ChEMBL_1670149 (CHEMBL4020037) Competitive inhibition of rabbit muscle glycogen phosphorylase-a in presence of varying levels of glucose-1-phosphate and constant concentration of glycogen and AMP preincubated for 15 mins with AMP and glycogen followed by glucose-1-phosphate addition by Lineweaver-Burk plot method
- ChEMBL_1670148 (CHEMBL4020036) Competitive inhibition of rabbit muscle glycogen phosphorylase-b in presence of varying levels of glucose-1-phosphate and constant concentration of glycogen and AMP preincubated for 15 mins with AMP and glycogen followed by glucose-1-phosphate addition by Lineweaver-Burk plot method
- ChEMBL_1288272 (CHEMBL3117346) Inhibition of mouse EAP using p-nitrophenyl phosphate as substrate after 30 mins by chemiluminescence assay
- ChEMBL_1500295 (CHEMBL3588719) Inverse agonist activity at rat ghrelin receptor expressed in HEK293 cells by inositol phosphate turnover assay
- ChEMBL_1520498 (CHEMBL3624737) Binding affinity to Grb7-SH2 domain (unknown origin) without using phosphate buffer by SPR analysis
- ChEMBL_153322 (CHEMBL760476) Inhibition of Purine nucleoside phosphorylase was evaluated against the enzyme from calf spleen in 50 mM phosphate
- ChEMBL_153323 (CHEMBL873308) Inhibition of Purine nucleoside phosphorylase was evaluated against the enzyme from calf spleen in 1 mM phosphate
- ChEMBL_153324 (CHEMBL882291) Inhibition of Purine nucleoside phosphorylase was evaluated against the enzyme from calf spleen in 50 mM phosphate
- ChEMBL_1538843 (CHEMBL3737418) Inhibition of GPAT1 (unknown origin) by CPM assay using glycerol-3-phosphate and oleoyl-CoA as substrate
- ChEMBL_1546979 (CHEMBL3748082) Binding affinity to Salmonella typhi glucose-1-phosphate cytidylyl-transferase assessed as dissociation constant by spectrophotometry
- ChEMBL_1619017 (CHEMBL3861186) Inhibition of rabbit muscle glycogen phosphorylase b using alpha-D-glucose-1-phosphate as substrate
- ChEMBL_1651766 (CHEMBL4001021) Inhibition of recombinant human ATX using bisP-nitrophenyl phosphate as substrate measured after 30 mins
- ChEMBL_1767106 (CHEMBL4202353) Inhibition of recombinant human GST-tagged PTP1B catalytic domain using para-nitrophenyl phosphate as substrate
- ChEMBL_2168507 (CHEMBL5053566) Inhibition of human SHIP2 (419 to 732 residues) expressed in Escherichia coli by malachite green phosphate assay
- ChEMBL_216946 (CHEMBL822915) Inhibitory potency for brewer's yeast alpha-glucosidase in 0.1 M sodium phosphate buffer at pH 6.55.
- ChEMBL_29964 (CHEMBL640398) 50% inhibition of human placental alkaline phosphatase in the presence of 10 mM p-nitrophenyl phosphate as substrate
- ChEMBL_361236 (CHEMBL871298) Antagonist activity at human recombinant GnRH receptor assessed as inhibition of GnRH-stimulated inositol phosphate accumulation
- ChEMBL_361237 (CHEMBL871299) Antagonist activity at rat pituitary GnRH receptor assessed as inhibition of GnRH-stimulated inositol phosphate accumulation
- ChEMBL_399839 (CHEMBL910318) Inhibition of non-phosphorylated form of rabbit muscle glycogen phosphorylase in presence of glycogen and 45 mM phosphate
- ChEMBL_399840 (CHEMBL910859) Inhibition of non-phosphorylated form of rabbit muscle glycogen phosphorylase in presence of 6.17 mM glycogen and phosphate
- ChEMBL_424432 (CHEMBL910711) Antagonist activity at human NK3 receptor expressed in CHO cells assessed as inhibition of inositol phosphate generation
- ChEMBL_424463 (CHEMBL912506) Activity at human NK3 receptor assessed as inhibition of senktide-stimulated inositol phosphate generation in CHO cells
- ChEMBL_440404 (CHEMBL890718) Agonist activity at mGlu4 receptor expressed in HEK 293 cells assessed as effect on inositol phosphate production
- ChEMBL_440405 (CHEMBL890719) Agonist activity at mGlu6 receptor expressed in HEK 293 cells assessed as effect on inositol phosphate production
- ChEMBL_440406 (CHEMBL890720) Agonist activity at mGlu7 receptor expressed in HEK 293 cells assessed as effect on inositol phosphate production
- ChEMBL_440407 (CHEMBL890721) Agonist activity at mGlu8 receptor expressed in HEK 293 cells assessed as effect on inositol phosphate production
- ChEMBL_445954 (CHEMBL896247) Agonist activity at rat mGluR4 receptor expressed HEK293 cells assessed as effect on inositol phosphate production
- ChEMBL_445955 (CHEMBL896248) Agonist activity at rat mGluR6 receptor expressed HEK293 cells assessed as effect on inositol phosphate production
- ChEMBL_445956 (CHEMBL896249) Agonist activity at rat mGluR7 receptor expressed HEK293 cells assessed as effect on inositol phosphate production
- ChEMBL_445957 (CHEMBL896250) Agonist activity at rat mGluR8 receptor expressed HEK293 cells assessed as effect on inositol phosphate production
- ChEMBL_457690 (CHEMBL925013) Antagonist activity at human CRTH2 receptor assessed as inhibition of PGD2-induced signaling by inositol-phosphate assay
- ChEMBL_460661 (CHEMBL926735) Antagonist activity at rat mGluR1 expressed in cerebellar granule cells assessed as accumulation of [3H]inositol phosphate
- ChEMBL_590795 (CHEMBL1037633) Agonist activity at guinea pig NK3 receptor expressed in cells assessed as accumulation of [3H]inositol phosphate
- ChEMBL_64947 (CHEMBL675692) Compound was tested for inhibitory activity against EPSP(5-enolpyruvylshikimate 3-phosphate) synthase in Escherichia coli.
- ChEMBL_64955 (CHEMBL675698) Compound was tested for inhibitory activity against EPSP(5-enolpyruvylshikimate 3-phosphate) synthase in Escherichia coli.
- ChEMBL_64960 (CHEMBL676326) Inhibitory activity was evaluated against Escherichia coli EPSP(5-enolpyruvyl-shikimate-3-phosphate) Synthase
- ChEMBL_65086 (CHEMBL673143) Compound was tested for inhibitory activity against EPSP(5-enolpyruvylshikimate 3-phosphate) synthase in Escherichia coli.
- ChEMBL_65093 (CHEMBL673150) Tested for the inhibition against Escherichia coli 5-enolpyruvyl-shikimate-3-phosphate synthase versus phosphoenolpyruvate
- ChEMBL_65096 (CHEMBL670573) Compound was tested for inhibitory activity against EPSP(5-enolpyruvylshikimate 3-phosphate) synthase in Escherichia coli.
- ChEMBL_754327 (CHEMBL1799103) Inhibition of human recombinant PTP1B assessed as inhibition of para-nitrophenyl phosphate to para-nitrophenol conversion by spectrophotometry
- ChEMBL_798051 (CHEMBL1943106) Inhibition of PTP1B assessed as para-nitrophenyl phosphate catalyzed hydrolysis of para-nitrophenol by p-NPP assay
- ChEMBL_801146 (CHEMBL1948973) Inhibition of Mycobacterium tuberculosis PTP-A using p-nitrophenyl phosphate as substrate measured for 10 mins by spectrofluorimetry
- ChEMBL_831490 (CHEMBL2066218) Inhibition of GST-tagged human recombinant PTP1B using para-nitrophenyl phosphate as substrate by spectrophotometric analysis
- ChEMBL_862647 (CHEMBL2174120) Inhibition of Plasmodium falciparum glucose-6-phosphate dehydrogenase after 2 hrs by resazurin/diaphorasecoupled assay
- Competitive Inhibition Assay PNPase activity (guanosine to guanine and ribose 1-phosphate) was followed by measuring the decrease in absorbance at 268nm.
- ChEMBL_1630554 (CHEMBL3873260) Competitive inhibition of rabbit muscle glycogen phosphorylase-b assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen measured at 1 min time interval for 1 to 5 mins by Hill plot method
- ChEMBL_2027541 (CHEMBL4681699) Inhibition of active recombinant human N-terminal His-tagged 11-betaHSD1 expressed in Escherichia coli using cortisone, NADPH and glucose-6-phosphate incubated for 2 hrs by glucose-6-phosphate dehydrogenase based coupled HTRF immuno-competitive assay
- Inhibition Assay Pooled human liver microsome suspension (20 mg/mL) was diluted with phosphate buffer to obtain a 5 mg/mL suspension. A solution of NADPH was prepared in phosphate buffer at a concentration of 5 mM. Separate stock solutions of each substrate were prepared in DMSO:MeCN (50:50 v/v), mixed, and diluted in phosphate buffer to obtain a single solution containing each substrate at five times its experimentally determined Km concentration.
- ChEBML_143203 Agonistic activity was determined against human Neuromedin B receptor transfected in HEK293 cells as accumulation levels of inositol phosphate
- ChEBML_219283 Ability to inhibit yeast glyoxalase I enzyme in 0.05 M phosphate buffer, pH 6.6, 30 degree C
- ChEBML_32781 Dissociation constant in high affinity sites where the binding is in 50 mM phosphate buffer for Alpha-2 adrenergic receptor
- ChEBML_32783 Dissociation constant in low affinity sites where the binding is in 50 mM phosphate buffer for Alpha-2 adrenergic receptor
- ChEMBL_1361029 (CHEMBL3295407) Antagonist activity at human recombinant 5-HT2A receptor assessed as inhibition of serotonin-induced inositol phosphate accumulation
- ChEMBL_1361031 (CHEMBL3295409) Antagonist activity at human recombinant 5-HT2C receptor assessed as inhibition of serotonin-induced inositol phosphate accumulation
- ChEMBL_1363386 (CHEMBL3295521) Antagonist activity at human GPR103 receptor assessed as inhibition of inositol-1-phosphate production by cell-based assay
- ChEMBL_1436200 (CHEMBL3386562) Inhibition of Mycobacterium tuberculosis IspC using 1-deoxy-D-xylulose 5-phosphate as substrate by photometric analysis
- ChEMBL_1448918 (CHEMBL3375685) Inhibition of PHOSPHO1 phosphoethanolamine activity (unknown origin) assessed as amount of inorganic phosphate release after 30 mins
- ChEMBL_145032 (CHEMBL753726) Ability to inhibit OT-induced inositol phosphate accumulation was determined in CHO cells expressing the human OT receptor
- ChEMBL_1476391 (CHEMBL3430021) Inhibition of recombinant human PTP1B (1 to 299) expressed in Escherichia coli using p-nitrophenyl phosphate as substrate
- ChEMBL_1476723 (CHEMBL3429074) Agonist activity at rat mGluR2 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476724 (CHEMBL3429075) Agonist activity at rat mGluR4 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476726 (CHEMBL3429077) Agonist activity at rat mGluR6 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476727 (CHEMBL3429078) Agonist activity at rat mGluR7 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476728 (CHEMBL3429079) Agonist activity at rat mGluR8 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476729 (CHEMBL3429080) Agonist activity at rat mGluR1 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1476730 (CHEMBL3429081) Agonist activity at rat mGluR5 expressed in HEK293 cells assessed as induction of inositol phosphate production by HTRF assay
- ChEMBL_1520499 (CHEMBL3624738) Binding affinity to Grb7-SH2 domain (unknown origin) using 100 mM phosphate buffer by SPR analysis
- ChEMBL_1566144 (CHEMBL3787813) Inhibition of recombinant human PTP-1B expressed in Escherichia coli TB1 using p-nitrophenyl phosphate as substrate
- ChEMBL_1586211 (CHEMBL3819926) Inhibition of human recombinant PTP1B expressed in Escherichia coli using p-nitrophenyl phosphate as substrate by UV spectroscopy
- ChEMBL_162594 (CHEMBL768410) Inhibitory effect against protein-tyrosine phosphatase Lar, using p-nitrophenyl phosphate as substrate at pH 5.5.
- ChEMBL_1630545 (CHEMBL3873251) Competitive inhibition of rabbit muscle glycogen phosphorylase-b in presence of varying glucose-1-phosphate levels and NADP
- ChEMBL_1793242 (CHEMBL4265161) Competitive inhibition of Mycobacterium tuberculosis PtpA using p-nitrophenyl phosphate as substrate by Michaelis-Menten plot analysis
- ChEMBL_201317 (CHEMBL806167) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 1 expressed on CHO cell membranes
- ChEMBL_201334 (CHEMBL806183) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 3 expressed on CHO cell membranes
- ChEMBL_201335 (CHEMBL806184) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 4 expressed on CHO cell membranes
- ChEMBL_201454 (CHEMBL807599) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 5 expressed on CHO cell membranes
- ChEMBL_2130000 (CHEMBL4839429) Inhibition of SLK (unknown origin) assessed as transfer of radiolabelled phosphate group from ATP by reaction biology method
- ChEMBL_2130002 (CHEMBL4839431) Inhibition of CLK2 (unknown origin) assessed as transfer of radiolabelled phosphate group from ATP by reaction biology method
- ChEMBL_2130003 (CHEMBL4839432) Inhibition of GSK3alpha (unknown origin) assessed as transfer of radiolabelled phosphate group from ATP by reaction biology method
- ChEMBL_2130004 (CHEMBL4839433) Inhibition of GSK3beta (unknown origin) assessed as transfer of radiolabelled phosphate group from ATP by reaction biology method
- ChEMBL_304296 (CHEMBL830091) Effective concentration against sphingosine-1-phosphate receptor 1 determined by a [c-35S]-GTP binding assay
- ChEMBL_304306 (CHEMBL830101) Effective concentration against sphingosine 1-phosphate receptor 2 determined by a [c-35S]-GTP binding assay
- ChEMBL_304307 (CHEMBL830102) Effective concentration against sphingosine 1-phosphate receptor 3 determined by a [c-35S]-GTP binding assay
- ChEMBL_304308 (CHEMBL830103) Effective concentration against sphingosine 1-phosphate receptor 4 determined by a [c-35S]-GTP binding assay
- ChEMBL_304309 (CHEMBL830104) Effective concentration against sphingosine 1-phosphate receptor 5 determined by a [c-35S]-GTP binding assay
- ChEMBL_304324 (CHEMBL829296) Effective concentration to activate wild-type P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_306053 (CHEMBL833009) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 1 expressed on CHO cell membranes
- ChEMBL_306054 (CHEMBL833010) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 2 expressed on CHO cell membranes
- ChEMBL_306055 (CHEMBL833011) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 3 expressed on CHO cell membranes
- ChEMBL_306056 (CHEMBL833012) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 4 expressed on CHO cell membranes
- ChEMBL_306057 (CHEMBL833013) Inhibition of [33P]-S1P binding to human Sphingosine 1-phosphate receptor 5 expressed on CHO cell membranes
- ChEMBL_313020 (CHEMBL874214) Inhibition of PGD2-induced inositol phosphate formation at human chemoattractant receptor-homologous molecule expressed on TH2 cells
- ChEMBL_382280 (CHEMBL866784) Activity at rat CaSR in CHO cells assessed as inhibition of calcium ion-induced [3H]inositol phosphate accumulation
- ChEMBL_382560 (CHEMBL869768) Inhibition of kinesin ATPase activity by enzyme-linked inorganic phosphate detection assay with 5 mins pre-incubation
- ChEMBL_40068 (CHEMBL655664) Inhibitory effect against human CD45 tyrosine phosphatase using p-nitrophenyl phosphate substrate at a pH 7.0
- ChEMBL_447478 (CHEMBL896501) Inhibition of human GnRHR expressed in RBL cells assessed as inhibition of GnRH-stimulated [3H]inositol phosphate hydrolysis
- ChEMBL_481449 (CHEMBL1008707) Inhibition of lyase activity of rat DNA polymerase beta after 30 mins by deoxyribose phosphate excision assay
- ChEMBL_535725 (CHEMBL989742) Inhibition of Mycobacterium tuberculosis cloned 1-deoxy-D-xylulose-5-phosphate synthase expressed in Escherichia coli
- ChEMBL_543916 (CHEMBL1020351) Inhibition of recombinant calmodulin mediated bovine brain PDE1 activation assessed as effect on inorganic phosphate release by spectrophotometry
- ChEMBL_556099 (CHEMBL954231) Agonist activity at rat 5HT2A receptor expressed in mouse NIH3T3 cells assessed as stimulation of inositol phosphate accumulation
- ChEMBL_559825 (CHEMBL1020696) Binding affinity to PSMA in human LNCaP cells assessed as cell surface binding in phosphate-buffered saline buffer
- ChEMBL_559827 (CHEMBL1020698) Binding affinity to PSMA in human PC3 cells assessed as cell surface binding in phosphate-buffered saline buffer
- ChEMBL_600348 (CHEMBL1041066) Agonist activity at 5HT2A receptor expressed in HEK cells assessed as inhibition of serotonin-induced inositol phosphate accumulation
- ChEMBL_610374 (CHEMBL1070013) Inhibition of SHP1 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610375 (CHEMBL1070014) Inhibition of PTP1B expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610376 (CHEMBL1070015) Inhibition of HePTP expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610377 (CHEMBL1070016) Inhibition of Lyp expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610378 (CHEMBL1070017) Inhibition of FAP1 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610379 (CHEMBL1070018) Inhibition of CD45 expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610380 (CHEMBL1070019) Inhibition of LAR expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610381 (CHEMBL1070020) Inhibition of PTPalpha expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610382 (CHEMBL1070021) Inhibition of VHR expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610383 (CHEMBL1070022) Inhibition of Cdc14A expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_610384 (CHEMBL1070023) Inhibition of LMWPTP expressed in Escherichia coli assessed as inhibition of p-nitrophenyl phosphate hydrolysis at pH 7 by spectrophotometry
- ChEMBL_612567 (CHEMBL1074221) Activity at human 5HT2A receptor expressed in HEK293 cells assessed as [3H]inositol phosphate production by scintillation counting
- ChEMBL_634153 (CHEMBL1119160) Inverse agonist activity at human 5HT2A receptor expressed in HEK293 cells assessed as inhibition of inositol phosphate accumulation
- ChEMBL_662795 (CHEMBL1252534) Antagonist activity at human bradykinin B1 receptor in human MR5 cells assessed as [3H]inositol phosphate accumulation
- ChEMBL_801147 (CHEMBL1948974) Inhibition of Mycobacterium tuberculosis PTP-B using p-nitrophenyl phosphate as substrate measured for 10 mins by spectrofluorimetry
- ChEMBL_821949 (CHEMBL2039748) Inhibition of human Tdp1 using 3'-phosphate-(4-methylumbelliferone)-thymidine as substrate after 180 mins by spectrophotometry
- ChEMBL_886806 (CHEMBL2210497) Inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate after 30 mins by spectrophotometric analysis
- ChEMBL_962306 (CHEMBL2389407) Inhibition of human recombinant GST-tagged PTP1B using para-nitrophenyl phosphate as substrate after 2 mins
- ChEMBL_972706 (CHEMBL2410915) Agonist activity at human GPR142 transfected in HEK293 cells after 1 hr by inositol phosphate accumulation assay
- NMR (OBClip) The experimental studies were carried out in 20 mM sodium phosphate buffer at pH7.4, at a temperature of 298 K.
- PKA assay The activity of PKA, activated by cAMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.
- UV/VIS The experimental studies were carried out in 20 mM sodium phosphate buffer at pH7.4, at a temperature of 298 K.
- cGPK assay The activity of cGPK, activated by cGMP, is measured by its ability to transfer phosphate from [gamma-32P]ATP to histone.
- ChEMBL_1630548 (CHEMBL3873254) Competitive inhibition of His6-tagged human liver glycogen phosphorylase-a expressed in Escherichia coli BL21 Gold (DE3) assessed as release of inorganic phosphate using varying levels of glucose-1-phosphate and constant concentration of AMP and glycogen measured after 1 min by Dixon plot method
- ChEBML_201314 Binding affinity to human Sphingosine 1-phosphate receptor 1 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201315 Binding affinity to human sphingosine 1-phosphate receptor 1 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201322 Binding affinity to human sphingosine 1-phosphate receptor 2 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201331 Binding affinity to human sphingosine 1-phosphate receptor 3 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201448 Binding affinity to human sphingosine 1-phosphate receptor 4 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201449 Binding affinity to human sphingosine 1-phosphate receptor 4 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_1288117 (CHEMBL3110577) Competitive inhibition Trypanosoma brucei PFK using fructose-6-phosphate as substrate by Line-weaver Burk plot analysis
- ChEMBL_143203 (CHEMBL752220) Agonistic activity was determined against human Neuromedin B receptor transfected in HEK293 cells as accumulation levels of inositol phosphate
- ChEMBL_1458873 (CHEMBL3368772) Antagonist activity at alphavbeta1 integrin receptor in HEK293 cells after 4 hrs using p-nitrophenyl phosphate by colorimetry
- ChEMBL_1458874 (CHEMBL3368773) Antagonist activity at alphavbeta3 integrin receptor in HEK293 cells after 4 hrs using p-nitrophenyl phosphate by colorimetry
- ChEMBL_1458875 (CHEMBL3368774) Antagonist activity at alphavbeta5 integrin receptor in HEK293 cells after 4 hrs using p-nitrophenyl phosphate by colorimetry
- ChEMBL_1458878 (CHEMBL3368777) Antagonist activity at alpha5beta1 integrin receptor in K562 cells after 4 hrs using p-nitrophenyl phosphate by colorimetry
- ChEMBL_1511315 (CHEMBL3606220) Inhibition of recombinant human GAPDH assessed as oxidative phosphorylation of D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate by spectrophotometric method
- ChEMBL_1548346 (CHEMBL3755948) Competitive inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate by spectrophotometry based Lineweaver-Burk plot
- ChEMBL_1554437 (CHEMBL3767568) Competitive inhibition of rabbit muscle glycogen phosphorylase b by Lineweaver-Burk plot analysis in presence of glucose 1-phosphate
- ChEMBL_1586208 (CHEMBL3819923) Inhibition of human recombinant PTP1B catalytic domain using p-nitrophenyl phosphate as substrate by micro plate reader method
- ChEMBL_162595 (CHEMBL772431) Inhibitory effect against human Protein-tyrosine phosphatase Lar using p-nitrophenyl phosphate substrate at a pH 7.0
- ChEMBL_1793241 (CHEMBL4265160) Non-competitive inhibition of Mycobacterium tuberculosis PtpA using p-nitrophenyl phosphate as substrate by Michaelis-Menten plot analysis
- ChEMBL_1812157 (CHEMBL4311617) Binding affinity to recombinant human CA2 in pH 7 sodium phosphate buffer at 37 degC by isothermal titration calorimetry
- ChEMBL_2014923 (CHEMBL4668501) Inhibition of SHP2 catalytic domain (unknown origin) using p-nitrophenyl phosphate as substrate incubated for 10 to 15 mins
- ChEMBL_216862 (CHEMBL816861) In vitro inhibition of the c-src tyrosine kinase activity in A431 membranes using angiotensin II as phosphate acceptor as substrate
- ChEMBL_2184903 (CHEMBL5096985) Inhibition of human SHP2 catalytic domain expressed in Escherichia coli using p-nitrophenyl phosphate as substrate by spectrophotometric method
- ChEMBL_2230346 (CHEMBL5144118) Inhibition of human IDO1 in potassium phosphate buffer at pH 6.5 using L-tryptophan as substrate incubated for 8 mins
- ChEMBL_226175 (CHEMBL847372) In vitro inhibition of the v-abl tyrosine kinase activity in A431 membranes using angiotensin II as phosphate acceptor as substrate
- ChEMBL_29963 (CHEMBL640397) Concentration required for 50% inhibition of human leukocyte alkaline phosphatase in the presence of 10 mM p-nitrophenyl phosphate as substrate
- ChEMBL_29968 (CHEMBL642161) Concentration required for 50% inhibition of human leukocyte alkaline phosphatase in the presence of 10 mM p-nitrophenyl phosphate as substrate
- ChEMBL_304388 (CHEMBL830038) Effective concentration required for the activation of wild-type P2Y1 receptors expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_30932 (CHEMBL647741) Inhibitory constant against adenosine deaminase in 0.05 M sodium phosphate buffer at pH 7.0 was determined before heating
- ChEMBL_30933 (CHEMBL647742) Inhibitory constant against adenosine deaminase in 0.05 M sodium phosphate buffer at pH 7.0, was determined before heating
- ChEMBL_32781 (CHEMBL644527) Dissociation constant in high affinity sites where the binding is in 50 mM phosphate buffer for Alpha-2 adrenergic receptor
- ChEMBL_32783 (CHEMBL644529) Dissociation constant in low affinity sites where the binding is in 50 mM phosphate buffer for Alpha-2 adrenergic receptor
- ChEMBL_48440 (CHEMBL662105) Effective concentration for the stimulation of Inositol Phosphate accumulation in CHO cells expressing wild-type Cholecystokinin type B receptor
- ChEMBL_495152 (CHEMBL1008931) Antagonist activity at human GnRH receptor expressed in RBL1 cells assessed as inhibition of GnRH-stimulated inositol phosphate production
- ChEMBL_565878 (CHEMBL959301) Activation of human glucokinase by glucose-6-phosphate dehydrogenase coupled continuous spectrophotometric assay in presence of 2.5 mM glucose
- ChEMBL_565879 (CHEMBL959302) Activation of human glucokinase by glucose-6-phosphate dehydrogenase coupled continuous spectrophotometric assay in presence of 10 mM glucose
- ChEMBL_634094 (CHEMBL1118878) Competitive reversible inhibition of Trypanosoma brucei PFK expressed in Escherichia coli using fructose-6-phosphate by spectrophotometry analysis
- ChEMBL_642245 (CHEMBL1176954) Inhibition of 6x-histidine-tagged human recombinant ATX expressed in HEK293 cells using bisP-nitrophenyl phosphate as a substrate
- ChEMBL_740875 (CHEMBL1763976) Inhibition of squalene synthase assessed as conversion of [3H]farnesyl phosphate to [3H]squalene after 10 mins by scintillation counting
- ChEMBL_749305 (CHEMBL1785240) Inhibition of Trypanosoma cruzi recombinant glycosomal GAPDH expressed in Escherichia coli using GAPDH and D-glyceraldehyde-3-phosphate
- ChEMBL_751030 (CHEMBL1786815) Non-competitive inhibition of Mycobacterium tuberculosis PTPA using p-nitrophenyl phosphate as substrate by Lineweaver-Burk plot analysis
- ChEMBL_829769 (CHEMBL2061288) Inhibition of MKP3 using 3-O-Methylfluorescein phosphate as substrate incubated for 10 mins prior to substrate addition by fluorometry
- ChEMBL_829770 (CHEMBL2061289) Inhibition of PTP1B using 3-O-Methylfluorescein phosphate as substrate incubated for 10 mins prior to substrate addition by fluorometry
- ChEMBL_829772 (CHEMBL2061291) Inhibition of PTPMT1 using 3-O-Methylfluorescein phosphate as substrate incubated for 10 mins prior to substrate addition by fluorometry
- ChEMBL_885380 (CHEMBL2215682) Activation of recombinant rat glucokinase assessed measuring rate of glucose 6-phosphate formation using G6PDH/NADP coupling
- ChEMBL_885387 (CHEMBL2216128) Activation of recombinant human glucokinase assessed measuring rate of glucose 6-phosphate formation using G6PDH/NADP coupling
- ChEMBL_930438 (CHEMBL3074004) Inhibition of snake venom phosphodiesterase using bis-(para-nitrophenyl)phosphate as substrate after 30 min by spectrophotometric analysis
- ChEMBL_936606 (CHEMBL2319005) Binding affinity to recombinant Tdp1 (unknown origin) using 3'-phosphate(GATCTAAAAGACTT) as substrate by surface plasmon resonance analysis
- ChEMBL_945952 (CHEMBL2341026) Binding affinity to GST-tagged human TNF-alpha in 10 mM citrate-phosphate at pH 6.5 by fluorescence assay
- Inhibition Assay (piHA-Dm) After kinetic analysis with HPA, piHA-Dm was tested as an inhibitor of ten additional enzymes. The conditions are listed as follows: Agrobacterium faecalis β-glucosidase (2.5 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate pH 7, 4 mM PNP β-gal); Bos taurus β-galactosidase (6.9 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM PNP β-gal); S. cerevisiae α-glucosidase (5 × 10^-3 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Human maltase-glucoamylase (8.1 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Mouse maltase-glucoamylase (8.1 × 10^-2 mg/mL enzyme, 50 mM sodium phosphate pH 7, 1 mM α-glc); Butyrivibrio fibrisolvens α-amylase (1.7 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate 100 mM NaCl pH 7, 1 mM CNPG3); Aspergillus oryzae α-amylase (1.9 × 10^-4 mg/mL enzyme, 50 mM sodium phosphate 100 mM NaCl pH 7, 1 mM CNPG3); Porcine pancreatic α-amylase (6.7 × 10^-3 mg/mL enzyme, 50 mM sodium phosphate 100 mM N
- ChEBML_138273 Effective concentration for HMPA (human muscarinic inositol phosphate accumulation) activity measured in CHO cells expressing Muscarinic acetylcholine receptor M1
- ChEBML_201324 Binding affinity towards human sphingosine 1-phosphate receptor 2 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201333 Binding affinity towards human sphingosine 1-phosphate receptor 3 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEBML_201450 Binding affinity towards human sphingosine 1-phosphate receptor 4 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_1283725 (CHEMBL3108305) Inhibition of Escherichia coli thymidine phosphorylase using thymidine 5' mono phosphate as substrate after 10 mins by spectrophotometric analysis
- ChEMBL_1436199 (CHEMBL3386561) Inhibition of Escherichia coli M15 recombinant IspC using 1-deoxy-D-xylulose 5-phosphate as substrate by photometric analysis
- ChEMBL_1458876 (CHEMBL3368775) Antagonist activity at alphavbeta6 integrin receptor in human HT29 cells after 4 hrs using p-nitrophenyl phosphate by colorimetry
- ChEMBL_1462475 (CHEMBL3398878) Inhibition of STEP (unknown origin) pre-incubated for 10 mins before addition of p-nitrophenyl phosphate substrate in absence of GSH
- ChEMBL_147734 (CHEMBL759351) Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 11
- ChEMBL_147744 (CHEMBL753284) Agonist activity by measuring inositol phosphate accumulation in 1321N1 human astrocytoma cells stably expressing human P2Y purinoceptor 2
- ChEMBL_1508846 (CHEMBL3603395) Inhibition of PIP5K1C (unknown origin) using D-myo-phosphatidylinositol 4-phosphate substrate and ATP by ADP-Glo kinase Assay
- ChEMBL_154380 (CHEMBL759143) Tested functionally in vitro for inducing 50% of the maximum alkaline phosphate activity (Transactivation) against Peroxisome proliferator activated receptor gamma
- ChEMBL_1548348 (CHEMBL3755950) Non-competitive inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate by spectrophotometry based Lineweaver-Burk plot
- ChEMBL_162602 (CHEMBL771000) Inhibitory effect against human Protein-tyrosine phosphatase alpha (PTPalpha) using p-nitrophenyl phosphate substrate at a pH 7.0
- ChEMBL_162603 (CHEMBL771315) Inhibitory effect against human protein-tyrosine phosphatase alpha (PTPalpha), using p-nitrophenyl phosphate substrate at pH 5.5.
- ChEMBL_1821937 (CHEMBL4321597) Inhibition of HK2 (unknown origin) using glucose-6-phosphate dehydrogenase as substrate preincubated for 10 mins followed by substrate addition
- ChEMBL_201314 (CHEMBL800921) Binding affinity to human Sphingosine 1-phosphate receptor 1 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201322 (CHEMBL806172) Binding affinity to human sphingosine 1-phosphate receptor 2 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201323 (CHEMBL806173) Binding affinity to human sphingosine 1-phosphate receptor 2 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201331 (CHEMBL806180) Binding affinity to human sphingosine 1-phosphate receptor 3 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201448 (CHEMBL873237) Binding affinity to human sphingosine 1-phosphate receptor 4 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201451 (CHEMBL807596) Binding affinity to human sphingosine 1-phosphate receptor 5 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_201452 (CHEMBL807597) Binding affinity to human sphingosine 1-phosphate receptor 5 expressed in CHO cells was determined by using [33P]-S1P as radioligand
- ChEMBL_2014921 (CHEMBL4668499) Inhibition of recombinant His6-fused SHP2 catalytic domain (unknown origin) using p-nitrophenyl phosphate as substrate by spectrophotometric analysis
- ChEMBL_2014922 (CHEMBL4668500) Inhibition of recombinant His6-fused SHP1 catalytic domain (unknown origin) using p-nitrophenyl phosphate as substrate by spectrophotometric analysis
- ChEMBL_2107526 (CHEMBL4816201) Competitive inhibition of G6PD (unknown origin) using glucose-6-phosphate and varying concentrations of NADP+ as substrate by Michaelis-Menten analysis
- ChEMBL_2161850 (CHEMBL5046711) Displacement of sphingosine D-erythro [3-3H] I- phosphate from human S1P2 receptor expressed in CHO cell membrane
- ChEMBL_303616 (CHEMBL828871) In vitro binding affinity of compound towards rat metabotropic glutamate receptor 5 was determined using inositol phosphate hydrolysis assay
- ChEMBL_304373 (CHEMBL829015) Effective concentration to activate human Y203F mutant strain P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_304374 (CHEMBL829016) Effective concentration to activate human Y273A mutant strain P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_304375 (CHEMBL829017) Effective concentration to activate human Y273F mutant strain P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_304376 (CHEMBL829018) Effective concentration to activate human Y306A mutant strain P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_304377 (CHEMBL829019) Effective concentration to activate human Y306F mutant strain P2Y1 receptor expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_306712 (CHEMBL833036) Concentration required for 50% growth inhibition of rat metabotropic glutamate receptor 5 was determined using inositol phosphate hydrolysis assay
- ChEMBL_310796 (CHEMBL835642) Inhibition of (Thr,Nle)-CCK-9 -induced inositol phosphate production in COS-7 cells expressing human CCK2 receptor
- ChEMBL_312857 (CHEMBL874936) Inhibition of (Thr,Nle)-CCK-9-induced inositol phosphate production in COS-7 cells expressing human CCK2 receptor
- ChEMBL_49009 (CHEMBL662889) Inhibitory activity tested against cell division cycle 25A (assay using p-nitro phenyl phosphate (pNPP) as substrate)
- ChEMBL_584072 (CHEMBL1058313) Activation of flag-tagged recombinant human liver glucokinase expressed in Escherichia coli assessed as glucose-6-phosphate dehydrogenase by spectrophotometry
- ChEMBL_607910 (CHEMBL1066238) Inhibition of thymidine phosphorylase in T cell lymphomas of Sprague-Dawley rat in presence of 100 uM thymidine and 200 uM phosphate
- ChEMBL_63519 (CHEMBL873581) Ability to block the ET-1-induced hydrolysis of inositol phosphate in Endothelin B receptor of chinese hamster ovary(CHO) cells.
- ChEMBL_727296 (CHEMBL1687390) Displacement of biotinylated phosphatidylinositol-3,4,5-phosphate from mouse AKT1 PH domain by surface plasmon resonance competitive binding assay
- ChEMBL_790928 (CHEMBL1926476) Inhibition of GST-tagged human recombinant PTP1B assessed as dephosphorylation of para-nitrophenyl phosphate after 10 mins by spectrophotometric analysis
- ChEMBL_829771 (CHEMBL2061290) Inhibition of human PTEN using 3-O-Methylfluorescein phosphate as substrate incubated for 10 mins prior to substrate addition by fluorometry
- ChEMBL_840475 (CHEMBL2089751) Inhibition of human recombinant PTP1B using p-nitrophenyl phosphate as substrate assessed as p-nitrophenol release after 30 mins
- ChEMBL_848287 (CHEMBL2149376) Inhibition of human recombinant LAR expressed in Escherichia coli TB1 using p-nitrophenyl phosphate as substrate after 1 hr
- ChEMBL_848288 (CHEMBL2149377) Mixed type inhibition of human recombinant PTP-1B expressed in Escherichia coli TB1 using p-nitrophenyl phosphate as substrate
- ChEMBL_848289 (CHEMBL2149378) Non-competitive inhibition of human recombinant PTP-1B expressed in Escherichia coli TB1 using p-nitrophenyl phosphate as substrate
- ChEMBL_860250 (CHEMBL2169191) Agonist activity at human GPR142 expressed in HEK293 cells assessed as inositol phosphate accumulation after 1 hr by scintillation counting
- ChEMBL_983901 (CHEMBL2433511) Inhibition of LAR (unknown origin) assessed as p-nitrophenyl phosphate conversion to p-nitrophenol after 10 mins by spectrophotometric analysis
- ChEMBL_983902 (CHEMBL2433512) Inhibition of SHP2 (unknown origin) assessed as p-nitrophenyl phosphate conversion to p-nitrophenol after 10 mins by spectrophotometric analysis
- ChEMBL_983903 (CHEMBL2433513) Inhibition of CD45 (unknown origin) assessed as p-nitrophenyl phosphate conversion to p-nitrophenol after 10 mins by spectrophotometric analysis
- ChEMBL_983904 (CHEMBL2433514) Inhibition of PTPbeta (unknown origin) assessed as p-nitrophenyl phosphate conversion to p-nitrophenol after 10 mins by spectrophotometric analysis
- ChEMBL_983905 (CHEMBL2433515) Inhibition of TCPTP (unknown origin) assessed as p-nitrophenyl phosphate conversion to p-nitrophenol after 10 mins by spectrophotometric analysis
- Glycogen Phosphorylase Enzyme Assay The enzymatic inhibition of phosphorylase activity was monitored using microplate reader (Bio-Rad). In brief, GPa activity was measured in the direction of glycogen synthesis by the release of phosphate from glucose-1-phosphate. Each compound was dissolved in DMSO and diluted at different concentrations for IC50 determination. The enzyme was added into the buffer containing compound, glucose-1-phosphate, and 1 mg/mL glycogen in 96-well microplates (costar). After the addition of ammonium molybdate and malachite green, reactions were run at 22 deg C for 25 min. And then the phosphate absorbance was measured at 655 nm. The IC50 values were estimated by fitting the inhibition data to a dose-dependent curve using a logistic derivative equation.
- ChEMBL_105574 (CHEMBL709771) Agonist activity against Metabotropic glutamate receptor 1 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation
- ChEMBL_105891 (CHEMBL717752) Agonist activity against Metabotropic glutamate receptor 2 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation
- ChEMBL_106545 (CHEMBL715490) Agonist activity against Metabotropic glutamate receptor 4 expressed in HEK 293 cells was evaluated by measuring total inositol phosphate accumulation
- ChEMBL_1353018 (CHEMBL3270626) Inhibition of Mycobacterium smegmatis DNA gyrase B ATPase activity assessed as inorganic phosphate release using ATP as substrate by colorimetric analysis
- ChEMBL_152658 (CHEMBL761584) Kinetic constant Apparent binding constant (Ki`) for the inhibition of papain conducted in 0.1 M phosphate, pH 6.8, at 30 degree C
- ChEMBL_1586212 (CHEMBL3819927) Inhibition of human recombinant TCPTP (1 to 341 residues) expressed in Escherichia coli using p-nitrophenyl phosphate as substrate by UV spectroscopy
- ChEMBL_1590325 (CHEMBL3830364) Competitive inhibition of rat S1PL in presence of D(+)-erythro-sphinganine-1-phosphate measured after 15 mins by Dixon plot analysis
- ChEMBL_162283 (CHEMBL770981) Inhibitory effect against recombinant human protein-tyrosine phosphatase 1B (PTP1B), using p-nitrophenyl phosphate substrate at pH 5.5.
- ChEMBL_1720812 (CHEMBL4135812) Inhibition of recombinant human GST-tagged PTP1B catalytic domain expressed in Escherichia coli BL21 using para-nitrophenyl phosphate as substrate
- ChEMBL_1856573 (CHEMBL4357302) Inhibition of Listeria monocytogenes NAD kinase assessed as suppression of reduced NADP formation by yeast glucose-6-phosphate dehydrogenase coupled assay
- ChEMBL_1924392 (CHEMBL4427348) Activation of human recombinant glucokinase using 5 mM glucose as substrate in presence of NAD+ by glucose 6-phosphate dehydrogenase coupled assay
- ChEMBL_1970364 (CHEMBL4603182) Inhibition of human recombinant AKR1B1 assessed as D,L-glyceraldehyde reduction incubated in sodium phosphate buffer at pH 6.2 in presence of NADPH
- ChEMBL_2016972 (CHEMBL4670550) Inhibition of actin-activated bovine heart muscle myosin 2 assessed as phosphate level incubated for 6 to 24 mins by spectrophotometric method
- ChEMBL_207128 (CHEMBL805112) Inhibitory effect against T cell protein tyrosine phosphatase (TC-PTP) using p-nitrophenyl phosphate as substrate at pH 7.0
- ChEMBL_2107522 (CHEMBL4816197) Non-competitive inhibition of G6PD (unknown origin) using glucose-6-phosphate and varying concentrations of NADP+ as substrate by Michaelis-Menten analysis
- ChEMBL_223552 (CHEMBL845858) Inhibitory effect against recombinant human PTP1B (Protein Tyrosine phosphatase 1B) using p-nitrophenyl phosphate substrate at pH 5.5
- ChEMBL_2273984 Inhibition of Staphylococcus aureus recombinant FTsZ preincubated for 10 mins followed by compound addition measured after 30 mins by Cytophos phosphate assay
- ChEMBL_304441 (CHEMBL832489) Effective concentration required for the activation of human R287A mutant strain P2Y1 receptors expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_304451 (CHEMBL832499) Effective concentration required for the activation of human T221A mutant strain P2Y1 receptors expressed in COS-7 cells for the accumulation of inositol phosphate
- ChEMBL_34910 (CHEMBL648446) Inhibitory constant was evaluated against Angiotensin I converting enzyme from rabbit lungs at pH 8 in TRIS phosphate/borate buffer
- ChEMBL_552859 (CHEMBL958064) Agonist activity at human P2Y6 receptor expressed in human 1321N1 cells assessed as [3H]inositol phosphate accumulation by scintillation proximity assay
- ChEMBL_552861 (CHEMBL958066) Agonist activity at human P2Y2 receptor expressed in human 1321N1 cells assessed as [3H]inositol phosphate accumulation by scintillation proximity assay
- ChEMBL_552863 (CHEMBL958068) Agonist activity at human P2Y4 receptor expressed in human 1321N1 cells assessed as [3H]inositol phosphate accumulation by scintillation proximity assay
- ChEMBL_685005 (CHEMBL1286523) Inhibition of human full-length Eg5 ATPase activity expressed in Escherichia coli assessed as release of inorganic phosphate after 30 mins
- ChEMBL_790077 (CHEMBL1925467) Inhibition of PI3K subunit p110alpha assessed as formation of phosphatidylinositide-3-phosphate product formation after 30 mins by fluorescence polarization assay
- ChEMBL_790079 (CHEMBL1925469) Inhibition of PI3K subunit p110beta assessed as formation of phosphatidylinositide-3-phosphate product formation after 30 mins by fluorescence polarization assay
- ChEMBL_790080 (CHEMBL1925470) Inhibition of PI3K subunit p110delta assessed as formation of phosphatidylinositide-3-phosphate product formation after 30 mins by fluorescence polarization assay
- ChEMBL_804877 (CHEMBL1953925) Inhibition of Streptococcus pneumoniae acetyltransferase activity of GlmU using acetyl-CoA and glucosamine-1-phosphate after 30 mins by Ellman's method
- ChEMBL_823683 (CHEMBL2044998) Inhibition of human recombinant PDE4B2 using fluorescein 3',5'-cyclic phosphate after 60 mins by IMAP fluorescence polarization assay
- ChEMBL_823684 (CHEMBL2044999) Inhibition of human recombinant PDE4D3 using fluorescein 3',5'-cyclic phosphate after 60 mins by IMAP fluorescence polarization assay
- ChEMBL_829768 (CHEMBL2061287) Inhibition of PTP-beta receptor using 3-O-Methylfluorescein phosphate as substrate incubated for 10 mins prior to substrate addition by fluorometry
- ChEMBL_852005 (CHEMBL2157130) Inhibition of His-tagged Cdc25A expressed in the Escherichia coli BL21(DE3) using p-nitrophenyl phosphate as substrate for 80 mins by spectrophotometry
- ChEMBL_899964 (CHEMBL3061740) Inhibition of recombinant Homo sapiens (human) Hsp90 alpha ATPase activity assessed as inorganic phosphate release by malachite green colorimetric method
- EGFR assay The activity of EGFR, preactivated with EGF, is measured by its ability to transfer terminal phosphate from [gamma-32P]ATP to poly(GAT) substrate.
- Malachite Green Assay A colorimetric assay for the release of inorganic phosphate upon hydrolysis of ATP was used to determine the potency of Hsp 90 inhibitor against enzyme.
- PKC assay The activity of PKC, activated by phosphatidylerine and Ca2+, is measured by its ability to transfer phosphate from [gamma-32P]ATP to lysine-rich histone.